|
| Status |
Public on Jan 01, 2014 |
| Title |
P3M3-1 |
| Sample type |
RNA |
| |
|
| Source name |
Adult LHM, whole fly
|
| Organism |
Drosophila melanogaster |
| Characteristics |
population base: LHM
tissue: whole body
developmental stage: adult
Sex: female
female.type: Prom
female.population: Prom3
male.type: Mono
male.population: Mono3
interaction: P.M
label: P3M3
batch: 1
|
| Treatment protocol |
From an LHM base population, we created 8 replicate populations and maintained them under experimental evolution: 4 populations were allowed to mate only once every generation (monogamy), and the other 4 were kept under the standard mating protocol (promiscuous). After 46 generations, we crossed males and females within the same population and with individuals of the opposite treatment. Mated female flies were frozen 6 h after mating.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen) extraction and RNeasy (Qiagen) purification.
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip IVT Labeling Kit, standard manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 with GCOS software.
|
| Description |
Female from promiscous population crossed with male from monogamous population.
|
| Data processing |
Probesets pre-processed with RMA, as implemented in R/Bioconductor package affy.
|
| |
|
| Submission date |
Jun 27, 2013 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Edward H Morrrow |
| Organization name |
University of Sussex
|
| Department |
Life Sciences
|
| Lab |
Morrow
|
| Street address |
Science Park Road
|
| City |
Brighton |
| ZIP/Postal code |
BN1 9QG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE48385 |
Post-mating expression of female Drosophila melanogaster genomes experimentally evolved under different post-copulatory sexual selection regimes |
|
