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Series GSE48385 Query DataSets for GSE48385
Status Public on Jan 01, 2014
Title Post-mating expression of female Drosophila melanogaster genomes experimentally evolved under different post-copulatory sexual selection regimes
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary By combining an experimental evolution approach with genomic techniques, we investigated the effects of seminal fluid on female gene expression. In our study, we experimentally manipulated the mating system in replicate populations of D. melanogaster, by removing post-copulatory sexual selection, with the aim of testing differences in short term post-mating reaction of females evolved under different mating strategies. We show that monogamous females suffer decreased fecundity, regardless of the type of male they were mated with, and that their post-mating gene expression profiles differ significantly from promiscuous females, involving 1141 transcripts (9% of the genes tested). These transcripts are active in several tissues, mainly ovaries, neural tissues, midgut and spermathecae, and are involved in metabolic processes, reproduction and signaling pathways. Our results provide a list of candidate genes responsible for the decrease in female fecundity in the absence of post-copulatory sexual selection, and demonstrate how the female post-mating response can evolve under different mating systems over relatively short time frames.
 
Overall design From an LHM base population, we created 8 replicate populations and maintained them under experimental evolution: 4 populations were allowed to mate only once every generation (monogamy), and the other 4 were kept under the standard mating protocol (promiscuous). After 46 generations, we crossed males and females within the same population and with individuals of the opposite treatment. Mated female flies were frozen 6 h after mating and RNA extracted. Two biological replicates per cross per population (2x2x8=32 samples).
 
Contributor(s) Innocenti P, Morrow EH
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Submission date Jun 27, 2013
Last update date May 04, 2018
Contact name Edward H Morrrow
Organization name University of Sussex
Department Life Sciences
Lab Morrow
Street address Science Park Road
City Brighton
ZIP/Postal code BN1 9QG
Country United Kingdom
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (32)
GSM1176833 M1M1-1
GSM1176834 M1M1-2
GSM1176835 M1P1-1
Relations
BioProject PRJNA209893

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48385_RAW.tar 62.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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