|
| Status |
Public on Dec 31, 2013 |
| Title |
W45-ox-1 |
| Sample type |
RNA |
| |
|
| Source name |
4th leaves at 4-leaf stage
|
| Organism |
Oryza sativa |
| Characteristics |
genotype/variation: WRKY45-ox
tissue: 4th leaves at 4-leaf stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol and cleaned up with an RNeasy plant mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
The RNAs (400-ng aliquots) were labeled with a Low RNA Input Linear Amplification/Labeling Kit (Agilent Technologies)
|
| |
|
| Hybridization protocol |
Agilent protocol G4140-90040
|
| Scan protocol |
Slides were scanned immediately after washing on Agilent Scanner model G2505B and software G2565BA using default parameters
|
| Data processing |
The scanned images were analyzed with Feature Extraction software (version 9.1; Agilent Technologies) using default parameters.
|
| |
|
| Submission date |
Jun 21, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Hiroshi Takatsuji |
| E-mail(s) |
takatsuh@affrc.go.jp
|
| Organization name |
National Institute of Agrobiological Sciences
|
| Department |
Division of Plant Sciences
|
| Lab |
Plant Disease Resistance Research Unit
|
| Street address |
2-1-2 Kannondai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8602 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8852 |
| Series (1) |
| GSE48202 |
The genes upregulated in WRKY45-ox rice relative to Nipponbare |
|
