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Status |
Public on Dec 31, 2013 |
Title |
The genes upregulated in WRKY45-ox rice relative to Nipponbare |
Organism |
Oryza sativa |
Experiment type |
Expression profiling by array
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Summary |
WRKY45-overexpressing (WRKY45-ox) rice plants are extremely resistant to rice blast and bacterial leaf-blight diseases. To search for the genes that are potentially responsible for the strong resistance, we performed genome-wide gene expression analysis in WRKY45-ox rice. A microarray analysis using Agilent rice oligo microarray for approximately 42,000 rice genes identified 1,664 genes that are upregulated in WRKY45-ox rice compared with non-transformant rice cv. Nipponbare (NB). Analysis of the genes with altered expression in WRKY45-ox rice by a Gene ontology program (Agri GO; http://bioinfo.cau.edu.cn/agriGO/) revealed that several genes in the biosynthetic pathway for diterpenoid phytoalexins, for which anti-blast fungus activities have been reported, were upregulated in WRKY45-ox rice.
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Overall design |
Nipponbare rice (Oryza sativa) and WRKY45-ox rice were analyzed in three biological replicates.
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Contributor(s) |
Akagi A, Takatsuji H |
Citation missing |
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Submission date |
Jun 21, 2013 |
Last update date |
Jan 09, 2014 |
Contact name |
Hiroshi Takatsuji |
E-mail(s) |
takatsuh@affrc.go.jp
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Organization name |
National Institute of Agrobiological Sciences
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Department |
Division of Plant Sciences
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Lab |
Plant Disease Resistance Research Unit
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Street address |
2-1-2 Kannondai
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City |
Tsukuba |
State/province |
Ibaraki |
ZIP/Postal code |
305-8602 |
Country |
Japan |
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Platforms (1) |
GPL8852 |
Agilent-015241 Rice Gene Expression 4x44K Microarray (Probe name version) |
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Samples (6)
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Relations |
BioProject |
PRJNA209195 |