|
| Status |
Public on Nov 04, 2013 |
| Title |
genomic DNA from differentiated, NLRP7 knockdown H9 hESC, replicate A |
| Sample type |
genomic |
| |
|
| Source name |
differentiated_NLRP7 knockdown H9 hESC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H9
cell type: Cultured hESC
genotype/variation: NLRP7 knockdown
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from cultured cells using Gentra Puregene DNA extraction Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
samples were hybridized on to 2 different arrays, this sample was done on array 1
H9-6A
|
| Data processing |
non_normalized_array*.txt contains unmethylated and methylated signal intensities
Illumina Genome Studio Methylation data were imported into the Illumina Genome Studio software and converted to a Partek Project File (.ppj) with the Partek Methylation Module
GenomeStudio Data Analysis Software’s Gene Expression Module (GSGX) Version 1.1.0
|
| |
|
| Submission date |
Apr 03, 2013 |
| Last update date |
Nov 04, 2013 |
| Contact name |
Sangeetha Kaveri Mahadevan |
| Organization name |
Baylor College of Medicine
|
| Department |
Obsterics and Gynecology
|
| Lab |
Ignatia Van den Veyver
|
| Street address |
1250 Moursund St, Room 1025
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE45727 |
NLRP7 promotes trophoblast lineage differentiation, YY1 binding and alters CpG methylation |
|
