|
| Status |
Public on Jun 27, 2013 |
| Title |
Bn-F2_28_days_old_leaf |
| Sample type |
SRA |
| |
|
| Source name |
Brassica napus leaf
|
| Organism |
Brassica napus |
| Characteristics |
cell type: resynthesized
passages: 4 weeks
strain: leaf
|
| Growth protocol |
Plants were cultivated in climate chambers at 25 °C, a 16 h light: 8h dark photoperiod, and 70% relative humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNAiso Plus reagent (Takara), followed by mRNA purification with oligo-dT-attached magnetic beads, cDNA was synthesized using Oligo (dT) as primer. Quality control was performed with Agilent Bioanalyser.
The DGE libraries were prepared using Illumina Gene Expression Sample Prep Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.4
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence
Supplementary_files_format_and_content: text files including tag copy numbers and TPM value of distinct sequence tags of each library
|
| |
|
| Submission date |
Jan 02, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jinjin Jiang |
| E-mail(s) |
jinjin.jiang@yahoo.cn
|
| Organization name |
Yangzhou University
|
| Street address |
Wenhui East Road No.12
|
| City |
Yangzhou |
| ZIP/Postal code |
225009 |
| Country |
China |
| |
|
| Platform ID |
GPL15579 |
| Series (1) |
| GSE43246 |
Gene expression profilling of six species of Brassica and early four generation of resynthesized B. napus (F1-F4) |
|
| Relations |
| SRA |
SRX213692 |
| BioSample |
SAMN01881838 |
