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Series GSE43246 Query DataSets for GSE43246
Status Public on Jun 27, 2013
Title Gene expression profilling of six species of Brassica and early four generation of resynthesized B. napus (F1-F4)
Organisms Brassica juncea; Brassica napus; Brassica nigra; Brassica rapa; Brassica oleracea; Brassica carinata
Experiment type Expression profiling by high throughput sequencing
Summary Analysis of the different gene expression profiles of natural and resynthesized Brassica polyploids with Illumina deep sequencing technology could help to improve our knowledge of polyploid genome evolution. We obtained approximately 6 million sequence tags per sample,and 6018254, 5930726, 6022170, 5950123, 5991210, 5798939, 5823113, 5772449,5858527 and 5657697 clean tags were obtained in libraries of B. rapa, B. oleracea, B. napus-F1, B. napus-F2, B. napus-F3, B. napus-F4, natural B. napus, B. nigra, B. juncea and B. carinata, respectively.16574, 15970, 22059, 18155, 16479, 18196, 17448, 13867, 19424 and 16645 genes of B. rapa genome were unambigously mapped by sequence tags of these ten DGE libraries, respectively. Differentially expressed genes during polyploidization were broadly discovered by comparing the tetraploids with their progenitors.
 
Overall design mRNA obtained from young leaves of 28-days-old seedlings were compared during polyploidization.
 
Contributor(s) Jiang J, Wang Y
Citation(s) 25623840, 23369045
Submission date Jan 02, 2013
Last update date Aug 13, 2019
Contact name Jinjin Jiang
E-mail(s) jinjin.jiang@yahoo.cn
Organization name Yangzhou University
Street address Wenhui East Road No.12
City Yangzhou
ZIP/Postal code 225009
Country China
 
Platforms (6)
GPL15579 Illumina HiSeq 2000 (Brassica napus)
GPL16360 Illumina HiSeq 2000 (Brassica oleracea)
GPL16440 Illumina HiSeq 2000 (Brassica rapa)
Samples (10)
GSM1059161 Br_28_days_old_leaf
GSM1059162 Bo_28_days_old_leaf
GSM1059163 Bn-F1_28_days_old_leaf
Relations
BioProject PRJNA185137
SRA SRP017725

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43246_RAW.tar 8.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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