|
| Status |
Public on Jan 01, 2015 |
| Title |
Methylation in colon mucosa 398 |
| Sample type |
genomic |
| |
|
| Source name |
colon mucosa 398
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 16
tissue: Colon mucosa
disease state: normal
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from colon mucosa samples using QIAamp DNA Mini Kit (Qiagen, Valencia, CA) starting at step 5 of the Tissue Protocol following bead beating and Qiagen TissueLyser treatment. All samples were quality tested with PicoGreen prior to proceeding with the microarrays.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to the Illumina Infinium HumanMethylation450 BeadChip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner settings
|
| Description |
Normal colon mucosa sample
|
| Data processing |
GenomeStudio software version 2010.3.0.30128
Average beta values were normalized to internal array controls as implemented in GenomeStudio
|
| |
|
| Submission date |
Dec 14, 2012 |
| Last update date |
Jan 01, 2015 |
| Contact name |
R. Alan Harris |
| E-mail(s) |
rharris1@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE42921 |
DNA methylation in a validation cohort of colon mucosa samples from individuals with Crohns' disease or ulcerative colitis and normal controls |
|
