|
Status |
Public on Oct 10, 2012 |
Title |
temperature: 25-25 C - rep2 |
Sample type |
RNA |
|
|
Source name |
Leaves & Stems (temperature: 25-25 C)
|
Organism |
Vitis vinifera |
Characteristics |
germplasm name: Cabernet Sauvignon organism part: Leaves & Stems cell type: all_leaf_cells development stage: leaf development stages ('PO:0001050') growth media: peat/perlite/soil temperature: 25-25 C
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extraction
|
Label |
biotin
|
Label protocol |
Affymetrix one-cycle target labeling
|
|
|
Hybridization protocol |
Affymetrix vitis vinifera Genechips
|
Scan protocol |
CNB Grapegen Affymetrix scanning
|
Data processing |
The MAS5.0 Normalization was performed using the justMAS function in the simpleaffy package of Bioconductor (www.bioconductor.org, Version: 2.12.1). The target value is 500 for all cases. The code was developed using R2.4.1 to 2.13.0, Bioconductor 1.9 to 2.12.1, and simpleaffy 2.8.0. to 2.28.0 The CDF files were downloaded from the Bioconductor website at http://www.bioconductor.org/packages/release/AffymetrixChip.html.
|
|
|
Submission date |
Oct 09, 2012 |
Last update date |
Oct 10, 2012 |
Contact name |
PLEXdb Curator, Sudhansu Dash |
Organization name |
Iowa State University
|
Department |
Virtual Reality Application Center
|
Lab |
PLEXdb
|
Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
City |
Ames |
State/province |
IA |
ZIP/Postal code |
50011 |
Country |
USA |
|
|
Platform ID |
GPL1320 |
Series (1) |
GSE41423 |
Transcriptomic analysis during heat stress and after the following recovery in grapevine (Vitis vinifera L.) leaves |
|