germplasm name: Cabernet Sauvignon organism part: Leaves & Stems cell type: all_leaf_cells development stage: leaf development stages ('PO:0001050') growth media: peat/perlite/soil temperature: 45 C
Extracted molecule
total RNA
Extraction protocol
Total RNA extraction
Label
biotin
Label protocol
Affymetrix one-cycle target labeling
Hybridization protocol
Affymetrix vitis vinifera Genechips
Scan protocol
CNB Grapegen Affymetrix scanning
Data processing
The MAS5.0 Normalization was performed using the justMAS function in the simpleaffy package of Bioconductor (www.bioconductor.org, Version: 2.12.1). The target value is 500 for all cases. The code was developed using R2.4.1 to 2.13.0, Bioconductor 1.9 to 2.12.1, and simpleaffy 2.8.0. to 2.28.0 The CDF files were downloaded from the Bioconductor website at http://www.bioconductor.org/packages/release/AffymetrixChip.html.
Submission date
Oct 09, 2012
Last update date
Oct 10, 2012
Contact name
PLEXdb Curator, Sudhansu Dash
Organization name
Iowa State University
Department
Virtual Reality Application Center
Lab
PLEXdb
Street address
1023 Crop Genomes Informatics Lab (Iowa State University)
