|
| Status |
Public on Nov 21, 2016 |
| Title |
TET proteins regulate lineage specification and TCR-mediated expansion of iNKT cells (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
TET proteins oxidize 5-methylcytosine to 5-hydroxymethylcytosine and further oxidation products in DNA. Here we report that simultaneous deletion of Tet2 and Tet3 in mouse double-positive thymocytes resulted in dysregulated development and proliferation of invariant natural killer T (iNKT) cells. Tet2-Tet3-double-deficient (DKO) iNKT cells displayed pronounced skewing towards the NKT17 lineage, with increased DNA methylation and impaired expression of genes encoding the key lineage-specifying factors T-bet and ThPOK. Transfer of purified Tet2-Tet3 DKO iNKT cells into immunocompetent recipient mice resulted in uncontrolled expansion dependent on the nonclassical MHC protein CD1d, which presents lipid antigens to iNKT cells. Our data indicate that TET proteins regulate iNKT cell fate by ensuring proper development and maturation and suppressing aberrant T cell antigen receptor (TCR)-mediated proliferation.
|
| |
|
| Overall design |
DKO vs. wild type
|
| |
|
| Contributor(s) |
Tsagaratou A, Rao A |
| Citation(s) |
27869820 |
| Submission date |
Mar 12, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Sini Rautio |
| Organization name |
Aalto University
|
| Street address |
Konemiehentie 2
|
| City |
Espoo |
| ZIP/Postal code |
02100 |
| Country |
Finland |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (19)
|
|
| This SubSeries is part of SuperSeries: |
| GSE66834 |
TET proteins regulate lineage specification and TCR-mediated expansion of iNKT cells |
|
| Relations |
| SRA |
SRP056118 |
| BioProject |
PRJNA282753 |
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