NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE60060 Query DataSets for GSE60060
Status Public on Apr 30, 2015
Title miRNA expression profiles from neurogenic differentiation of mesenchymal stem cells under conditioned microenvironments
Platform organism synthetic construct
Sample organism Rattus norvegicus
Experiment type Non-coding RNA profiling by array
Summary Background: Studies suggested that mesenchymal stem cells (MSCs) have intrinsic neurogenic potential and can be differentiated into neural stem cell/ neural progenitor cells (NPCs) under specific microenvironment. Manipulation of growth factors is one of the popular method to achieve trans-lineage differentiation of MSCs. Synergistic effect of epidermal growth factor (EGF) and fibroblast growth factor 2 (bFGF) have been widely identified as basic requirement for neural differentiation to take place. Insulin-like growth factor 1 (IGF-1) also known as somatomedin C is an important growth promoting protein during embryonic development which control numerous cellular responses and biological systems. Our recent study has found that the combination of EGF, bFGF and IGF-1 could significantly improved the growth and survivability of MSCs-derived NPCs. Therefore, to understand the genomic mechanism underlying the differentiation in vitro, we have studied the miRNAs profile of MSCs-derived NPCs under IGF-1 influenced conditioned microenvironments.
Objectives: To evaluate the effects of IGF-1 in trans-lineage differentiation of MSCs, we have induced MSCs into neural lineage in 3 groups; Group A (positive control) - EGF+bFGF, Group B (Treatment) - EGF+bFGF+IGF-1, and Group C (negative control/ untreated). To unravel the role of regulatory miRNAs involved in the early differentiation, we have performed detailed miRNA profiling for MSCs-derived NPCs at three time intervals (day 1, day 3 and day 5). The data has explored crucial miRNAs involved in early differentiation of MSCs into NPCs.
 
Overall design Stage specific MSCs-derived NPCs at Passage 1 were collected for total RNA extraction at three time-points (D1, D3 and D5) and hybridization on Affymetrix miRNA geneChip 2.0 arrays. Each experiment were repeated three times independently (Exp 1, Exp 2 and Exp 3). Group A served as positive control (EGF+bFGF), Group B as treatment (EGF+bFGF+IGF-1) and Group C without growth factor as negative control.
 
Contributor(s) Huat TJ, Khan AA, Abdullah JM, Jaafar H, Idris FM
Citation(s) 25938966
Submission date Aug 04, 2014
Last update date May 02, 2017
Contact name Amir Ali Khan
E-mail(s) amkhan@sharjah.ac.ae
Organization name University of Sharjah
Department Department of Applied Biology
Lab College of Sciences
Street address Emirates of Sharjah
City Sharjah
ZIP/Postal code PO Box 27272
Country United Arab Emirates
 
Platforms (1)
GPL14613 [miRNA-2] Affymetrix Multispecies miRNA-2 Array
Samples (27)
GSM1464425 Group A MSCs-derived NPCs (EGF+bFGF) at day 1, biological rep 1
GSM1464426 Group A MSCs-derived NPCs (EGF+bFGF) at day 1, biological rep 2
GSM1464427 Group A MSCs-derived NPCs (EGF+bFGF) at day 1, biological rep 3
Relations
BioProject PRJNA257399

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60060_RAW.tar 20.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap