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| Status |
Public on Feb 07, 2014 |
| Title |
Differential gene expression in 2 days old zebrafish after glucocerebrosidase (GBA1) morpholino-mediated knockdown |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression signature was explored to identify gene targets regulate by dysfunctional glucocerebrosidase function. We found 131 upregulated genes and 63 downregulated genes. Some of the most upregulated (PON3, PLOD1) and downregulated (gpx-1b and complemewnt c9) genes were analyzed by q-PCR demonstrating the correct differential gene expression signature among control and morpholino-microinjected fish.
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| Overall design |
Knockdown of GBA1 function was measured in 4 biological replicates at 2 days post fertilization after microinjection of GBA1 morpholino and control morpholino at one -cell stage embryos.
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| Contributor(s) |
Moro E, Zancan I, Bellesso S, Filocamo M |
| Citation(s) |
25326392 |
| Submission date |
Feb 06, 2014 |
| Last update date |
Oct 28, 2014 |
| Contact name |
Enrico Moro |
| E-mail(s) |
moroe@bio.unipd.it
|
| Phone |
+390498276341
|
| Organization name |
University of Padova
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| Department |
Molecular Medicine
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| Street address |
Via U. Bassi 58/B
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| City |
PADOVA |
| ZIP/Postal code |
35121 |
| Country |
Italy |
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| Platforms (1) |
| GPL6563 |
Agilent-015064 D. rerio (Zebrafish) Oligo Microarray 4x44K G2519F (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA237550 |
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