|
| Status |
Public on Jun 21, 2006 |
| Title |
Genomic responses to ectopic expression of DHR96 in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
DHR96 plays a role in regulating xenobiotic responses in Drosophila. Using a gain-of-function approach we test whether DHR96 is sufficient to affect detoxification genes in the absence of a xenobiotic insult.
Keywords: gain-of-function
|
| |
|
| Overall design |
A transgenic line that expresses a full-length DHR96 cDNA under heat-shock control was generated by standard P-element-mediated transformation, using w1118 as a host strain. Control flies are therefore w1118 without any transgene. Larvae were staged at the molt from L2 to L3, and heat-treated after 3 hours for one hour at 37.5 degrees (in vials). Finally, RNA was extracted 4 hours later.
|
| |
|
| Contributor(s) |
King-Jones K, Horner MA, Lam G, Thummel CS |
| Citation(s) |
16814731 |
| Submission date |
Jun 16, 2006 |
| Last update date |
May 04, 2018 |
| Contact name |
Kirst King-Jones |
| E-mail(s) |
kingjone@ualberta.ca
|
| Phone |
780-4928605
|
| Organization name |
University of Alberta
|
| Department |
Biological Sciences
|
| Lab |
King-Jones
|
| Street address |
77 University Campus
|
| City |
Edmonton |
| State/province |
AB |
| ZIP/Postal code |
T6H 5V3 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
|
| Samples (6)
|
| GSM115017 |
Ectopic expression of DHR96 (gain of function) control 1 |
| GSM115020 |
Ectopic expression of DHR96 (gain of function) control 2 |
| GSM115021 |
Ectopic expression of DHR96 (gain of function) control 3 |
| GSM115022 |
Ectopic expression of DHR96 (gain of function) gain 1 |
| GSM115023 |
Ectopic expression of DHR96 (gain of function) gain 2 |
| GSM115024 |
Ectopic expression of DHR96 (gain of function) gain 3 |
|
| Relations |
| BioProject |
PRJNA95349 |
Less...
More...