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| Status |
Public on Dec 11, 2013 |
| Title |
Alteration in transcript level in wheat alloplasmic lines |
| Organism |
Triticum aestivum |
| Experiment type |
Expression profiling by array
|
| Summary |
Alloplasmic lines provide a unique tool to study the nucleo-cytoplasmic interactions. Alloplasmic lines T183 and T195 were developed through the introgression of the cytoplasm from Aegilops uniaristata (T183) and Aegilops squarrosa (T195) in the nuclear background of Triticum aestivum cv. Chris. Alloplasmic line TH237 was produced introgressing the Hordeum chilense accession H7 cytoplasm into the nuclear background of Triticum aestivum accession T20. Fifty seeds for each sample in pots of 11 cm diameter and grown in controlled conditions under 600µE m-2 s–1 high light intensity in a daily regime of 12 h light at 22°C and 12 h darkness at 15°C. Plants were bulked from each pot and three biological replicate used for the transcriptomics Fully expanded second leaves were collected two weeks from sowing in the middle of the light period and used for transcriptomic analysis.
****PLEXdb (http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Cristina Crosatti. The equivalent experiment is TA49 at PLEXdb.
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| |
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| Overall design |
genotype: EU_T20(3-replications);
genotype: ALLO_TH237(3-replications);
genotype: EU_CHRIS(3-replications);
genotype: ALLO_183(3-replications);
genotype: ALLO_195(3-replications)
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| Web link |
http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=TA49
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| |
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| Contributor(s) |
Crosatti C, Cattivelli L |
| Citation(s) |
24320731 |
| Submission date |
Jul 01, 2013 |
| Last update date |
Dec 04, 2018 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
|
| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3802 |
[wheat] Affymetrix Wheat Genome Array |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA210140 |
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