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| Status |
Public on May 22, 2010 |
| Title |
Expression data from porcine hepatic and adipose tissue of males and females fed with two feeding levels |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
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| Summary |
Breed, gender and diet are factors affecting porcine metabolism. The aim of this study has been to investigate the gene expression patterns of the major sites for lipid metabolism, liver and fat, conditional on gender and on a moderate feeding restriction in Iberian pigs, as a model of obese porcine breed. Our results show that tissue effect account for more differentially expressed genes than gender or feeding restriction. The results obtained from the comparison between tissues support the studies showing adipose tissue is not only a fat-storage depot, we report a high number of upregulated genes in adipose tissue which represent relevant biological functions such as carbohydrate and energy metabolisms and endocrine function. Besides, key genes implicated in lipid metabolism are specifically overrepresented in liver or fat, particularly the differentially expressed genes related to fatty acid synthesis support previous studies showing that in pig, as in cattle or sheep, this process largely occurs in fat. We identified metabolic differences between genders such as oxidation capacity or response to toxins, reflected at gene expression level in liver but no in adipose tissue, contrarily to previous studies. Finally, our results seem to indicate that a moderate feeding restriction does not have large effects on liver or fat gene expression of obese pigs. Although the list of differentially expressed genes due to the effect of feeding restriction is limited, we could identify expression differences in genes related to antiageing mechanisms associated with feeding restriction as enhancement of immune response and anticoagulation and the balance between prosurvival and cell-death.
Breed, gender and diet are factors affecting porcine metabolism. The aim of this study has been to investigate the gene expression patterns of the major sites for lipid metabolism, liver and fat, conditional on gender and on a moderate feeding restriction in Iberian pigs, as a model of obese porcine breed. Our results show that tissue effect account for more differentially expressed genes than gender or feeding restriction. The results obtained from the comparison between tissues support the studies showing adipose tissue is not only a fat-storage depot, we report a high number of upregulated genes in adipose tissue which represent relevant biological functions such as carbohydrate and energy metabolisms and endocrine function. Besides, key genes implicated in lipid metabolism are specifically overrepresented in liver or fat, particularly the differentially expressed genes related to fatty acid synthesis support previous studies showing that in pig, as in cattle or sheep, this process largely occurs in fat. We identified metabolic differences between genders such as oxidation capacity or response to toxins, reflected at gene expression level in liver but no in adipose tissue, contrarily to previous studies. Finally, our results seem to indicate that a moderate feeding restriction does not have large effects on liver or fat gene expression of obese pigs. Although the list of differentially expressed genes due to the effect of feeding restriction is limited, we could identify expression differences in genes related to antiageing mechanisms associated with feeding restriction as enhancement of immune response and anticoagulation and the balance between prosurvival and cell-death.
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| Overall design |
16 liver and subcutaneous backfat samples from eight animals at slaughter, 211 days old, four males and four females, four under high feeding level and four under 20% restriction
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| Contributor(s) |
Fernández AI, Óvilo C, Fernández A, Fernández-Rodríguez A, Pérez-Montarelo D, Barragán C, López-Bote C, Toro MA, Rodríguez M, Silió L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 21, 2010 |
| Last update date |
May 03, 2013 |
| Contact name |
Ana I Fernandez |
| E-mail(s) |
avila@inia.es
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| Organization name |
INIA
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| Department |
Mejora Genética Animal
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| Street address |
Ctra. Coruña Km 7.5
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
| GPL3533 |
[Porcine] Affymetrix Porcine Genome Array |
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| Samples (16)
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| GSM545916 |
liver_male_High feeding level_animal 14 |
| GSM545917 |
liver_male_Low feeding level_animal 15 |
| GSM545918 |
liver_male_Low feeding level_animal 16 |
| GSM545919 |
liver_male_High feeding level_animal 17 |
| GSM545920 |
liver_female_Low feeding level_animal 18 |
| GSM545921 |
liver_female_High feeding level_animal 19 |
| GSM545922 |
liver_female_High feeding level_animal 20 |
| GSM545923 |
liver_female_Low feeding level_animal 21 |
| GSM545924 |
fat_male_High feeding level_animal 14 |
| GSM545925 |
fat_male_Low feeding level_animal 15 |
| GSM545926 |
fat_male_Low feeding level_animal 16 |
| GSM545927 |
fat_male_High feeding level_animal 17 |
| GSM545928 |
fat_female_Low feeding level_animal 20 |
| GSM545929 |
fat_female_High feeding level_animal 21 |
| GSM545930 |
fat_female_High feeding level_animal 22 |
| GSM545931 |
fat_female_Low feeding level_animal 23 |
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| Relations |
| BioProject |
PRJNA127241 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21952_RAW.tar |
29.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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