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| Status |
Public on Apr 18, 2019 |
| Title |
Free circular introns with an unusual branchpoint in neuronal projections |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
In eukaryotic cells, most introns are degraded soon after splicing in the nucleus but some persist either due to lack of splicing (detained/retained introns) or because they contain important functional elements, for example, sno/scaRNAs. Few introns are detectable outside the nucleus. To hunt for interesting new phenomena in cytoplasmic introns and splicing, we conducted a multimodal study of total RNA within projections (axons, dendrites, glial projections) of rat hippocampal neurons and discovered a class of free circular introns enriched in distal projections.
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| Overall design |
A variety of sequencing datasets from rat primary hippocampal neurons are provided: From physically separated cellular projections and whole-cell lysates, (a) rRNA-depleted total RNA-seq (5 biological replicates, each) and (b) polyA site sequencing or PAS-seq (3 biological replicates, each); from fractionated cytoplasmic lysate (c) ribosome profiling (3 biological replicates) and (d) polyA+ RNA-seq (3 biological replicates).
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| Contributor(s) |
Saini H, Eddy SR, Moore MJ |
| Citation(s) |
31697236 |
| Submission date |
Apr 16, 2019 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Harleen Saini |
| E-mail(s) |
harleen_saini@hms.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Lab |
Danesh Moazed
|
| Street address |
240 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (3) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
| GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA533159 |
| SRA |
SRP192787 |
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