|
| Status |
Public on Sep 21, 2017 |
| Title |
Determination of trastuzumab responsive microRNAs in SKBR3 and BT474 cells. |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
To develop gene-miRNA and pathway-miRNA networks in trastuzumab treatment, we performed a recent miRNA microarray profiling in trastuzumab treated/untreated SKBR3 and BT474 cell lines.
The cells were plated at a starting density of 2 millions in 100 mm cell culture dishes and then, they were treated with 6 ug/mL trastuzumab and PBS in two replicates. Total RNA was isolated with the TRIzol reagent (Invitrogen) according to the manufacturer's instructions.
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| Overall design |
RNA samples were isolated after 144h trastuzumab treatment. A total of 4 samples from BT474 cells (2 from trastuzumab treated and 2 from PBS treated) and 4 samples from SKBR3 cells (2 from trastuzumab treated and 2 from PBS treated) were analyzed.
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| Contributor(s) |
Cilek EE, Dedeoglu BG |
| Citation(s) |
28981542 |
| Submission date |
Sep 20, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Bala Gur Dedeoglu |
| Organization name |
Ankara University Biotechnology Institute
|
| Street address |
Dogol cad.
|
| City |
Ankara |
| ZIP/Postal code |
06110 |
| Country |
Turkey |
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| Platforms (1) |
| GPL18044 |
Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA408159 |
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