Robo3 roundabout guidance receptor 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Robo3provided by MGI
Official Full Name: roundabout guidance receptor 3provided by MGI
Primary source: MGI:MGI:1343102
See related: Ensembl:ENSMUSG00000032128 AllianceGenome:MGI:1343102
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rig1; Rbig1; Rig-1; Robo3a; Robo3b
Summary: Involved in axon midline choice point recognition. Acts upstream of or within axon guidance and neuron migration. Located in axon. Is expressed in several structures, including central nervous system; cranial nerve; future brain; head mesenchyme; and sensory organ. Orthologous to human ROBO3 (roundabout guidance receptor 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in CNS E11.5 (RPKM 18.7), cortex adult (RPKM 8.7) and 5 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A4; 9 20.76 cM

Exon count:: 30

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (37327341..37344730, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (37416045..37433436, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our findings identify a mechanism that YTHDF1-mediated translation of m6A-modified Robo3.1 mRNA controls pre-crossing axon guidance in spinal cord
Title: The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression.
This study show that this commissural neuron population includes spinal inhibitory neurons and sensory nociceptors and show that this population depends on Robo/Slit signaling yet crosses the dorsal midline in a Robo3-independent manner.
Title: Sensory and spinal inhibitory dorsal midline crossing is independent of Robo3.
These findings identify NELL2 as an axon guidance cue and establish Robo3 as a multifunctional regulator of pathfinding that simultaneously mediates NELL2 repulsion, inhibits Slit repulsion, and facilitates Netrin attraction to achieve a common guidance purpose.
Title: Operational redundancy in axon guidance through the multifunctional receptor Robo3 and its ligand NELL2.
results suggest that Robo3 evolution was key to sculpting the mammalian brain by converting a receptor for Slit repulsion into one that both silences Slit repulsion and potentiates Netrin attraction
Title: Signaling switch of the axon guidance receptor Robo3 during vertebrate evolution.
this study demonistrated that Robo3-driven axon midline crossing conditions functional maturation of a large commissural synapse.
Title: Robo3-driven axon midline crossing conditions functional maturation of a large commissural synapse.
Nonsense-mediated mRNA decay regulates Robo3.2 synthesis by inducing the degradation of Robo3.2 transcripts in axons that encounter the floor plate.
Title: Regulation of axon guidance by compartmentalized nonsense-mediated mRNA decay.
Rerouting climbing fibers to an ipsilateral projection reversed complex-spike modulation. Ptf1a::cre;Robo3(lox/lox) mice showed severe ataxia indicating the impact of climbing fi ber input on reciprocity of Purkinje cell fi ring during motor coordination.
Title: Climbing fiber input shapes reciprocity of Purkinje cell firing.
Data show that the gooseberry/gooseberry-neuro (gsb/gsb-n) transcription factor genes act to specify MP3 cell fate.
Title: Slit2 and Robo3 modulate the migration of GnRH-secreting neurons.
Posttranscriptional regulation of Robo3 mediated by Musashi-1 controls midline crossing of precerebellar neurons.
Title: Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
Visfatin through STAT3 activation enhances IL-6 expression that promotes endothelial angiogenesis.
Title: Regulation and function of the cytosolic viral RNA sensor RIG-I in pancreatic beta cells.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (1)
Targeted (4) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Robo3 (e-PCR), detects polymorphism
- UniSTS:479031

Robo3 (e-PCR), detects polymorphism
- UniSTS:479032

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within axon guidance	IDA Inferred from Direct Assay more info	PubMed
involved_in axon guidance	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon midline choice point recognition	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chemotaxis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within nervous system development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of axon guidance	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: roundabout homolog 3

Names: retinoblastoma inhibiting gene 1; retinoblastoma-inhibiting gene 1 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001164767.1 → NP_001158239.1 roundabout homolog 3

Status: VALIDATED

Source sequence(s)

AC138284

Consensus CDS

CCDS52770.1

UniProtKB/Swiss-Prot

A0A1L1SU85, D3Z4M6, Q9Z2I4

UniProtKB/TrEMBL

E9QPQ1

Related

ENSMUSP00000110690.2, ENSMUST00000115038.3

Conserved Domains (5) summary

cd05726
Location:361 → 450

Ig4_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

smart00408
Location:465 → 528

IGc2; Immunoglobulin C-2 Type

cd00063
Location:564 → 649

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:258 → 343

I-set; Immunoglobulin I-set domain

cl11960
Location:64 → 162

Ig; Immunoglobulin domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

37327341..37344730 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011242424.4 → XP_011240726.1 roundabout homolog 3 isoform X2

UniProtKB/TrEMBL

E9QPQ1

Conserved Domains (3) summary

cd05726
Location:361 → 450

Ig4_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd00063
Location:563 → 648

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cl11960
Location:64 → 162

Ig; Immunoglobulin domain
XM_006510107.5 → XP_006510170.1 roundabout homolog 3 isoform X1

UniProtKB/TrEMBL

E9QPQ1

Conserved Domains (3) summary

cd05726
Location:361 → 450

Ig4_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd00063
Location:564 → 649

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cl11960
Location:64 → 162

Ig; Immunoglobulin domain
XM_036154724.1 → XP_036010617.1 roundabout homolog 3 isoform X6

Conserved Domains (1) summary

cd00063
Location:51 → 136

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
XM_011242425.4 → XP_011240727.1 roundabout homolog 3 isoform X3

UniProtKB/TrEMBL

E9QPQ1

Conserved Domains (3) summary

cd05726
Location:319 → 408

Ig4_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd00063
Location:522 → 607

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cl11960
Location:22 → 120

Ig; Immunoglobulin domain
XM_011242427.4 → XP_011240729.1 roundabout homolog 3 isoform X5

See identical proteins and their annotated locations for XP_011240729.1

Conserved Domains (1) summary

cd00063
Location:51 → 136

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
XM_011242426.3 → XP_011240728.1 roundabout homolog 3 isoform X4

See identical proteins and their annotated locations for XP_011240728.1

Conserved Domains (1) summary

cd00063
Location:51 → 136

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

RNA

XR_004935349.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_011248.1: Suppressed sequence

Description

NM_011248.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.