GEO DataSets

Response of calvarial MC3T3-1b cells to osteogenic stimulus (beta-glycerophosphate (GP), ascorbic acid (AA) and bone morphogenic protein 2 (BMP-2)) for 0, 1 and 3 days. Notch, Wnt and TGF-β signaling pathways are activated in osteogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 growth protocol, 3 time sets

Platform:

GPL81

Series:

GSE1131

15 Samples

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(Submitter supplied) MC3T3 cells clone 1b were grown in alpha-MEM with 10% fetal calf serum (FCS, Gibco), 1% penicilin/streptomycin (Pen/Strep) and 1% L-Glutamine (L-Glu). For differentiation experiment, cells were seeded on 6 cm dishes (3x105cells/dish in 5 ml medium) and grownt to confluence for 3 days at 37C / 5% CO2. Cells were then stimulated with osteogenic stimulus (10 mM beta-glycerophosphate (GP, Sigma), 50 µM ascorbic acid (AA, Wako) and 1 mg/ml BMP-2 (Novartis)), or with 10mM GP alone for 1 or 3 days. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS679

Platform:

GPL81

15 Samples

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(Submitter supplied) To identify potential target genes regulated by BMP9 in MSCs, we used microarray to profile expression patterns of BMP9- vs. GFP-stimulated MSCs.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

6 Samples

Download data: CEL

(Submitter supplied) Vascular disruption following bony injury results in a hypoxic gradient within the wound microenvironment. Nevertheless, the effects of low oxygen tension on osteogenic precursors remain to be fully elucidated. In the present study, we investigated in vitro osteoblast and mesenchymal stem cell differentiation following exposure to 21% O(2) (ambient oxygen), 2% O(2) (hypoxia), and <0.02% O(2) (anoxia). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2635

2 Samples

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(Submitter supplied) Background: Osteoblast differentiation requires the coordinated stepwise expression of multiple genes. Histone deacetylase inhibitors (HDIs) accelerate the osteoblast differentiation process by blocking the activity of histone deacetylases (HDACs), which alter gene expression by modifying chromatin structure. We previously demonstrated that HDIs and HDAC3 shRNAs accelerate matrix mineralization and the expression of osteoblast maturation genes (e.g. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3002

Platform:

GPL1261

15 Samples

Download data: CEL

Analysis of MC3T3-E1 preosteoblasts treated with the histone deacetylase inhibitor (HDI) trichostatin A, MS-275, or valproic acid for 18 hours under osteogenic conditions. HDIs accelerate osteoblast maturation. Results provide insight into molecular mechanisms underlying osteoblast differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 agent sets

Platform:

GPL1261

Series:

GSE9247

15 Samples

Download data: CEL

(Submitter supplied) Aims of study: (1) To identify systemic differences in osteoarthritic (OA) bone that contribute to OA pathogenesis. (2) Identify novel osteoporotic (OP) bone-related disease genes. Study involved comparison of trabecular bone extracted from the intertrochanteric (IT) region of the proximal femur (PF) from OA, OP and normal/control (CTL) individuals. Bone was obtained from OA and OP individuals at surgery for total hip replacement and from CTL individuals at autopsy. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5391

39 Samples

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(Submitter supplied) Mesenchymal chondrosarcoma is a high-grade malignant neoplasm characterized by biphasic growth of poorly differentiated small round cells and well differentiated cartilage. Mesenchymal chondrosarcoma affects adolescents and young adults, and the HEY1-NCOA2 fusion gene is causally associated with most cases. Here we generate a mouse model for mesenchymal chondrosarcoma by introducing HEY1-NCOA2 into mouse embryonic chondrogenic progenitors (eSZ) followed by subcutaneous transplantation into nude mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21626

1 Sample

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL16417 GPL11180

46 Samples

Download data: CEL, TDF

(Submitter supplied) Mesenchymal chondrosarcoma is a high-grade malignant neoplasm characterized by biphasic growth of poorly differentiated small round cells and well differentiated cartilage. Mesenchymal chondrosarcoma affects adolescents and young adults, and the HEY1-NCOA2 fusion gene is causally associated with most cases. Here we generate a mouse model for mesenchymal chondrosarcoma by introducing HEY1-NCOA2 into mouse embryonic chondrogenic progenitors followed by subcutaneous transplantation into nude mice. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL16417

11 Samples

Download data: TDF

(Submitter supplied) Mesenchymal chondrosarcoma is a high-grade malignant neoplasm characterized by biphasic growth of poorly differentiated small round cells and well differentiated cartilage. Mesenchymal chondrosarcoma affects adolescents and young adults, and the HEY1-NCOA2 fusion gene is causally associated with most cases. Here we generate a mouse model for mesenchymal chondrosarcoma by introducing HEY1-NCOA2 into mouse embryonic chondrogenic progenitors followed by subcutaneous transplantation into nude mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11180

35 Samples

Download data: CEL

(Submitter supplied) Metazoans utilize a handful of highly conserved signaling pathways to create a signaling backbone that governs all stages of development, by providing spatial and temporal cues that influence gene expression. How these few signals have such a versatile developmental action is of significance to evolution, development, and disease. Their versatility likely depends upon the larger-scale network they form through integration. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL72

24 Samples

Download data: CEL

(Submitter supplied) BMP6 mediated osteoblast differentiation plays a key role in skeletal development and bone disease. Unfortunately, the signaling pathways regulated by BMP6 are largely uncharacterized due to both a lack of data and the complexity of the response. To better characterize the signaling pathways responsive to BMP6, we conducted a time series microarray study to track BMP6 induced osteoblast differentiation and mineralization.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13303

26 Samples

Download data: CEL

(Submitter supplied) Enhanced understanding of differential gene expression and biological pathways associated with distinct phases of intramembranous bone regeneration following femoral marrow ablation surgery will improve future advancements regarding osseointegration of joint replacement implants, biomaterials design, and bone tissue engineering. A rat femoral marrow ablation model was performed and genome-wide microarray data were obtained from samples at 1, 3, 5, 7, 10, 14, 28, and 56 days post-ablation, with intact bones serving as controls at Day 0. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

27 Samples

Download data: CEL

(Submitter supplied) In order to explore molecules whose expression is controlled by Slc39a13, we investigated gene expression profiling of primary chondrocyte isolated from wild-type and Slc39a13 knockout mice. Keywords: knockout vs wild-type

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3485

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) In order to explore molecules whose expression is controlled by Slc39a13, we investigated gene expression profiling of primary osteoblast isolated from wild-type and Slc39a13 knockout mice. Keywords: knockout vs wild-type

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL, CHP

Analysis of chondrocytes lacking the zinc transporter SLC39A13. Zinc is an essential trace element that is abundant in connective tissues. Results provide insight into the role of zinc in connective tissues.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE10556

6 Samples

Download data: CEL, CHP

(Submitter supplied) The regeneration of bone in large bone defects remains a challenge in orthopaedics. It may be possible that the difference in the adult height of mammals, and hence in endochondral bone formation, can serve to identify targets for bone regeneration. In line with this hypothesis, the intra-species disparity in adult height of Great Danes and Miniature Poodles was investigated at a transcriptional level. more...

Organism:

Canis lupus familiaris

Type:

Expression profiling by array

Platform:

GPL15184

22 Samples

Download data: TXT

(Submitter supplied) This study explores the profiles of genes expressed during conditional differentiation of inner ear hair cell precursors in vitro. The cell line UB/OC-1 was derived from mouse embryonic organs of Corti (Rivolta et al, Proc. R. Soc. Lond. B 265: 1595-1603, 1998) and it can be induced to differentiate upon a temperature shift. The study has been replicated in two separate populations of cells, named Exp A and Exp B. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS45 GDS51

Platforms:

GPL75 GPL76

32 Samples

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Gene expression profile of differentiation of inner ear hair cell precursors in vitro. A conditionally immortal cell line derived from the mouse cochlea, UB/OC-1, examined from 0 to 14 days.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 12 time sets

Platform:

GPL76

Series:

GSE36

16 Samples

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