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| Status |
Public on Jun 01, 2004 |
| Title |
Osteoblast differentiation experiment: BMP-2 induced osteogenesis in mouse MC3T3 cell line |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
MC3T3 cells clone 1b were grown in alpha-MEM with 10% fetal calf serum (FCS, Gibco), 1% penicilin/streptomycin (Pen/Strep) and 1% L-Glutamine (L-Glu). For differentiation experiment, cells were seeded on 6 cm dishes (3x105cells/dish in 5 ml medium) and grownt to confluence for 3 days at 37C / 5% CO2. Cells were then stimulated with osteogenic stimulus (10 mM beta-glycerophosphate (GP, Sigma), 50 µM ascorbic acid (AA, Wako) and 1 mg/ml BMP-2 (Novartis)), or with 10mM GP alone for 1 or 3 days. Control cells-day 0, medium. Experiment was done in triplicate. Total RNA was extracted, treated with DNAse, and purified according to the manufacturer’s protocol (RNeasy mini kit, QIAGEN). RNA samples were analyzed on oligonucleotide microarrays Affymetrix GeneChip® Murine Genome U74Av2.
Keywords: ordered
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| Contributor(s) |
Zamurovic N |
| Citation(s) |
15178686 |
| Submission date |
Mar 18, 2004 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Natasa Zamurovic |
| E-mail(s) |
natasa.zamurovic@pharma.novartis.com
|
| Phone |
+41 61 696 74 45
|
| Fax |
+41 61 696 38 49
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| Organization name |
Novartis Institutes for BioMedical Research
|
| Department |
Bone metabolism
|
| Street address |
|
| City |
Basel |
| ZIP/Postal code |
4002 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (15)
|
| GSM18546 |
MC3T3 cells day 0 medium prep 3. |
| GSM18547 |
MC3T3 cells day 1 GP prep 3. |
| GSM18548 |
MC3T3 cells day 1 GP/AA/BMP-2 prep 3. |
| GSM18549 |
MC3T3 cells day 3 GP prep 3. |
| GSM18550 |
MC3T3 cells day 3 GP/AA/BMP-2 prep 3. |
| GSM18551 |
MC3T3 cells day 0 medium prep 1. |
| GSM18552 |
MC3T3 cells day 1 GP prep 1. |
| GSM18553 |
MC3T3 cells day 1 GP/AA/BMP-2 prep 1. |
| GSM18554 |
MC3T3 cells day 3 GP prep 1. |
| GSM18555 |
MC3T3 cells day 3 GP/AA/BMP-2 prep 1. |
| GSM18556 |
MC3T3 cells day 0 medium prep 2. |
| GSM18557 |
MC3T3 cells day 1 GP prep 2. |
| GSM18558 |
MC3T3 cells day 1 GP/AA/BMP-2 prep 2. |
| GSM18559 |
MC3T3 cells day 3 GP prep 2. |
| GSM18560 |
MC3T3 cells day 3 GP/AA/BMP-2 prep 2. |
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| Relations |
| BioProject |
PRJNA87245 |
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