|
Status |
Public on Aug 08, 2013 |
Title |
tAML patient sample SJTAML005 |
Sample type |
RNA |
|
|
Source name |
RNA extracted from tumor cells
|
Organism |
Homo sapiens |
Characteristics |
disease state: therapy-related myeloid neoplasm (t-MN) chromosome 5q status: Chromosome 5q wild type cell type: mononuclear cell
|
Treatment protocol |
None
|
Growth protocol |
RNA was extracted from cryopreserved mononuclear cell preparations of diagnostic bone marrow or peripheral blood from t-MN cases
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 3’IVT Express protocol starting with 100 ng of total RNA (GeneChip 3’ IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix)
|
|
|
Hybridization protocol |
Following fragmentation, 5 microgram of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan system.
|
Data processing |
Data were background adjusted, normalized and summarized by the RMA method. The Bioconductor package was used for this analysis.
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|
|
Submission date |
Aug 08, 2012 |
Last update date |
Aug 08, 2013 |
Contact name |
Shann-Ching Chen |
E-mail(s) |
Shann-Ching.Chen@stjude.org
|
Organization name |
St. Jude Children's Research Hospital
|
Department |
Pathology
|
Street address |
262 Danny Thomas Place
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL13158 |
Series (1) |
GSE39991 |
Knockdown of Hnrnpa0, a del(5q) Gene, Alters Myeloid Cell Fate in Murine Cells through Regulation of AU-rich Transcripts |
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