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Sample GSM973471

Query DataSets for GSM973471

Status

Public on Sep 30, 2012

Title

Wild-type rice leaves 48 h after M.Oryzae infection replicate 1

Sample type

RNA

Source name

Wild-type rice leaves infected with M.Oryzae

Organism

Oryza sativa

Characteristics

genotype/variation: Wild-type
infection: M. oryzae
tissue: leaf

Treatment protocol

WT and OsWRKY28-overexpressing rice plants were infected with M. oryzae strain Ina86-137 or treated with water at the four-leaf stage and then fourth leaves were harvested from three plants for each samples at 48 h after treatments.

Growth protocol

Rice plants were grown in a chamber under a 14-h-light (28℃) and 10-h-dark (24℃) cycle in hydroponic culture.

Extracted molecule

total RNA

Extraction protocol

RNeasy Plant Mini Kit (Qiagen)

Label

Cy3

Label protocol

Low RNA input Linear Amplification/Labeling Kit (Agilent)

Hybridization protocol

Agilent protocol G4140-90040

Scan protocol

Agilent scanner model G2565

Description

Wild-type rice leaves infected with M.Oryzae
Biological replicate 1 of 3.

Data processing

After data was analyzed by Fearure Extraction software (version 11, Agilent), data normalization was performed using Partek Genomics Suite software (ver. 6.5; Partek software) by quantile normalization method.

Submission date

Jul 25, 2012

Last update date

Sep 30, 2012

Contact name

Kazunori Okada

E-mail(s)

ukokada@mail.ecc.u-tokyo.ac.jp

Phone

+81-3-5841-3070

Organization name

The University of Tokyo

Department

Biotechnology Research Center

Lab

Laboratory of Environmental Biochemistry

Street address

1-1-1, Yayoi, Bunkyo-ku

City

Tokyo

ZIP/Postal code

113-8657

Country

Japan

Platform ID

GPL8852

Series (1)

GSE39635

WRKY type transcription factor OsWRKY28 overexpression effect on rice leaves 48 h after Magnaporthe Oryzae infection

Data table header descriptions
ID_REF
VALUE	Normalized signal intensity (log 2)

Data table
ID_REF	VALUE
Os01g0100100\|COMBINER_EST\|CI448596\|0	10.7292
Os01g0100200\|mRNA\|AK059894\|CDS+3'UTR	3.16623
Os01g0100400\|mRNA\|AK101455\|CDS+3'UTR	5.25162
Os01g0100500\|mRNA\|AK067316\|CDS+3'UTR	12.1408
Os01g0100600\|mRNA\|AK121362\|CDS+3'UTR	11.8259
Os01g0100700\|mRNA\|AK059844\|CDS+3'UTR	11.3428
Os01g0100700\|mRNA\|AK121523\|CDS+3'UTR	9.79776
Os01g0100800\|mRNA\|AK122012\|CDS+3'UTR	10.4707
Os01g0100900\|COMBINER_EST\|CI015509\|0	13.2207
Os01g0101200\|mRNA\|AK067866\|CDS+3'UTR	13.4332
Os01g0101200\|mRNA\|AK104517\|CDS+3'UTR	16.2376
Os01g0101200\|mRNA\|AK104625\|CDS+3'UTR	16.1521
Os01g0101200\|mRNA\|AK104752\|CDS+3'UTR	16.0938
Os01g0101200\|mRNA\|AK119457\|CDS+3'UTR	7.96872
Os01g0101300\|COMBINER_EST\|CI016681\|6	8.07468
Os01g0101600\|mRNA\|AK099952\|CDS+3'UTR	7.12761
Os01g0101600\|mRNA\|AK103820\|CDS+3'UTR	12.1169
Os01g0101600\|mRNA\|AK122118\|CDS+3'UTR	12.0788
Os01g0101700\|COMBINER_EST\|CI525185\|3	14.0614
Os01g0101800\|mRNA\|AK103498\|CDS+3'UTR	8.57874

Total number of rows: 42477

Table truncated, full table size 1847 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM973471_US90500268_251524114600_S01_GE1_105_Dec08_1_2.txt.gz	7.6 Mb	(ftp)(http)	TXT
Processed data included within Sample table