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Sample GSM957830 Query DataSets for GSM957830
Status Public on Jun 20, 2013
Title NK.v2.Foxp3.DTR-.Sp.15hr.061011#1
Sample type RNA
 
Source name NK.v2.Foxp3.DTR-.Sp.15hr
Organism Mus musculus
Characteristics age: 7 to 10 weeks
exclusion markers: CD19- CD3-
tissue: Spleen
strain: NOD
phenotype markers: NKp46+
separation method: MoFlo Sorter
Sex: female
Treatment protocol BDC2.5/NOD.Foxp3-DTR were injection with DT and NK cells (CD19-CD3-Nkp46+) at various time points were collected. Target organs were harvested and prepared into single cell suspension, stained using fluorescent antibodies and sorted on a MOFLO to high purity (double sorted). RNA from those sorted cells was analyzed by affymetrix microarray.
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Jul 09, 2012
Last update date Jun 20, 2013
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (1)
GSE39197 Gene expression profiling of NK cells isolated from the pancreas of BDC TCR expressing NOD mice before or after Treg ablation by DTR. Also, NK cells cultured in vitro with activating cytokines IL12 and IL-18 with or without regulatory TGFb.

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 33.8415
10344622 190.9241
10344624 1099.3232
10344633 805.4255
10344637 450.3242
10344653 31.6277
10344658 877.8735
10344674 16.936
10344679 50.1164
10344707 675.0084
10344713 227.1901
10344719 131.8266
10344723 36.9404
10344725 78.7556
10344743 49.9885
10344750 312.5977
10344772 56.0455
10344789 353.8409
10344797 442.4743
10344799 472.9122

Total number of rows: 25194

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM957830_EA07068_145409_MOGENE-1_0-ST-V1_NK.V2.FOXP3.DTR-.SP.15HR.061011_1.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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