|
| Status |
Public on Jun 20, 2013 |
| Title |
NK.TRCC.Sp.T18.IL1218TGFb.062211#1 |
| Sample type |
RNA |
| |
|
| Source name |
NK.TRCC.Sp.T18.IL1218TGFb
|
| Organism |
Mus musculus |
| Characteristics |
age: 7 to 10 weeks
exclusion markers: CD19- CD3-
tissue: Spleen
strain: NOD
phenotype markers: NKp46+
separation method: MoFlo Sorter
Sex: male
|
| Treatment protocol |
NK cells (CD19-CD3-Nkp46+) were sorted on MOFLO from NOD mice, cultured in cRPMI in a 96 well plate and activated with a combination of IL-12 and IL-18 with or without regulatory TGFb overnight. Cells were analyzed using intracellular fluorescent staining for IFNg and analyzed on an LSRII machine and analyzed using FlowJo Software.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
|
| |
|
| Hybridization protocol |
Ambion WT Expression Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Data processing |
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Jul 09, 2012 |
| Last update date |
Jun 20, 2013 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE39197 |
Gene expression profiling of NK cells isolated from the pancreas of BDC TCR expressing NOD mice before or after Treg ablation by DTR. Also, NK cells cultured in vitro with activating cytokines IL12 and IL-18 with or without regulatory TGFb. |
|
