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Sample GSM953941 Query DataSets for GSM953941
Status Public on Jul 04, 2012
Title cervix_tumour_cs2_ac_2
Sample type RNA
 
Source name cervix tumour FFPE series 2 ac
Organism Homo sapiens
Characteristics tissue source: clinical cervical tumour sample
tissue type: tumour
storage: formalin-fixed paraffin-embedded (FFPE)
classification: adenocarcinoma (AC)
data series: 2
ffpe block storage duration time (yrs): 19
Growth protocol Cell lines were grown in DMEM + 10% FCS. Cell lines were classified as SCC or AC according to P63 protein expression
Extracted molecule total RNA
Extraction protocol RNA was extracted and Dnase treated using RecoverAll Total Nucleic Acid Isolation kit (Ambion).
Label FlashTag-Biotin-HSR
Label protocol Genisphere FlashTag Biotin HSR RNA labelling Kit
 
Hybridization protocol GeneChip Eukaryotic Hybridization Control Kit - Genisphere standard protocol
Scan protocol Affymetrix GeneChip scanner 3000 running GCOS software
Description CS2_AC2
Data processing Background adjustment and quantile normalisation using Affymetrix miRNA QC Tool (v.1.1.1.0)
probe group file: miRNA-2_0_v2.pgf
 
Submission date Jun 29, 2012
Last update date Jul 04, 2012
Contact name Jan Taylor
E-mail(s) catharine.west@manchester.ac.uk
Organization name Paterson Institute for Cancer Research
Department Bioinformatics
Street address Wilmslow Road
City Manchester
ZIP/Postal code M20 4BX
Country United Kingdom
 
Platform ID GPL14613
Series (2)
GSE39015 Stability of miRNA in FFPE tumour samples exhibiting degraded mRNA [Cervix samples miRNA]
GSE39067 Stability of miRNA in FFPE tumour samples exhibiting degraded mRNA

Data table header descriptions
ID_REF
VALUE Log 2 probeset expression estimates from quantile normalisation

Data table
ID_REF VALUE
AFFX-BioB-5_at 9.739891
AFFX-BioB-M_at 10.14672
AFFX-BioB-3_at 10.11088
AFFX-BioC-5_at 12.63155
AFFX-BioC-3_at 11.98624
AFFX-BioDn-5_at 13.58104
AFFX-BioDn-3_at 14.18926
AFFX-CreX-5_at 14.90633
AFFX-CreX-3_at 15.00813
AFFX-r2-Ec-c1-bioB-5_at 9.630278
AFFX-r2-Ec-c1-bioB-M_at 10.19232
AFFX-r2-Ec-c1-bioB-3_at 9.769065
AFFX-r2-Ec-c1-bioC-5_at 12.93794
AFFX-r2-Ec-c1-bioC-3_at 12.84194
AFFX-r2-Ec-c1-bioD-5_at 14.12669
AFFX-r2-Ec-c1-bioD-3_at 14.04321
AFFX-r2-P1-c1-cre-5_at 14.99264
AFFX-r2-P1-c1-cre-3_at 15.18873
AFFX-r2-Ec-c2-bioB-5_at 9.275036
AFFX-r2-Ec-c2-bioB-M_at 10.35093

Total number of rows: 20643

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM953941_CS2_ac_rep2_miRNA.CEL.gz 624.8 Kb (ftp)(http) CEL
Processed data included within Sample table

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