|
Status |
Public on Jul 04, 2012 |
Title |
cervix cell line Me180 |
Sample type |
RNA |
|
|
Source name |
cervix cell line Me180
|
Organism |
Homo sapiens |
Characteristics |
tissue source: cervix cell line tissue type: fresh storage: N/A classification: squamous cell carcinoma (SCC) data series: cell line ffpe block storage duration time (yrs): N/A
|
Growth protocol |
Cell lines were grown in DMEM + 10% FCS. Cell lines were classified as SCC or AC according to P63 protein expression
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted and Dnase treated using RecoverAll Total Nucleic Acid Isolation kit (Ambion).
|
Label |
FlashTag-Biotin-HSR
|
Label protocol |
Genisphere FlashTag Biotin HSR RNA labelling Kit
|
|
|
Hybridization protocol |
GeneChip Eukaryotic Hybridization Control Kit - Genisphere standard protocol
|
Scan protocol |
Affymetrix GeneChip scanner 3000 running GCOS software
|
Description |
CL_Me180
|
Data processing |
Background adjustment and quantile normalisation using Affymetrix miRNA QC Tool (v.1.1.1.0) probe group file: miRNA-2_0_v2.pgf
|
|
|
Submission date |
Jun 29, 2012 |
Last update date |
Jul 04, 2012 |
Contact name |
Jan Taylor |
E-mail(s) |
catharine.west@manchester.ac.uk
|
Organization name |
Paterson Institute for Cancer Research
|
Department |
Bioinformatics
|
Street address |
Wilmslow Road
|
City |
Manchester |
ZIP/Postal code |
M20 4BX |
Country |
United Kingdom |
|
|
Platform ID |
GPL14613 |
Series (2) |
GSE39015 |
Stability of miRNA in FFPE tumour samples exhibiting degraded mRNA [Cervix samples miRNA] |
GSE39067 |
Stability of miRNA in FFPE tumour samples exhibiting degraded mRNA |
|