|
| Status |
Public on Jul 31, 2012 |
| Title |
wt skel muscle, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Hind limb skeletal muscle from wt 11 days-old mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 11 days-old
tissue: skeletal muscle
genotype: wild-type, TK2+/+
|
| Growth protocol |
Official Swedish regulations for use and care of laboratory animals were followed throughout and the experimental protocol was approved by the local ethical commitee (S104-09, S135-11)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscle tissues were harvested immediatelly after we have sacrificed the mice. Total RNA was extracted using Rneasy kit (QIAGEN), according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Labelling was done using Ambion WT Expression kit (Life Technologies), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
|
| Scan protocol |
Automated with GeneTitan Instrument and Affymetrix GeneChip Command software (AGCC)
|
| Description |
Gene expression data from skeletal muscle of 11 days-old wild-type mouse
|
| Data processing |
Affymetrix Expression Console Software; Summarization: PLIER, Normalization: Global Median
|
| |
|
| Submission date |
Jun 04, 2012 |
| Last update date |
Jul 31, 2012 |
| Contact name |
João Amorim Paredes |
| E-mail(s) |
joao.paredes@ki.se
|
| Organization name |
Karolinska Institute
|
| Department |
Laboratory Medicine
|
| Lab |
Anna Karlsson
|
| Street address |
Karolinska Universitetssjukhuset Huddinge, F68
|
| City |
Huddinge |
| State/province |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE38449 |
Gene expression analysis in skeletal muscle and heart of 11 days-old TK2 knockout mice |
|
