|
| Status |
Public on Nov 30, 2012 |
| Title |
elc1∆1 |
| Sample type |
SRA |
| |
|
| Source name |
Yeast cells, elc1 deletion mutant
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: W303 elc1-delta::URA3
|
| Treatment protocol |
None.
|
| Growth protocol |
YPD 30 °C to mid-log phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the RNeasy Mini Kit (QIAGEN) and on-column DNase I digestion was performed. Samples were subjected to RiboZero and standard Illumina library preparation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
Base-calling was performed using Illumina CASAVA pipeline and standard parameters.
RNA-seq reads were aligned to the S288c reference R64 genome assembly using Novoalign V2.07.10 with default parameters.
wig files were generated using samtools depth.
Genome_build: Saccharomyces cerevisiae S288C reference genome R64 downloaded from SGD (http://www.yeastgenome.org/).
Supplementary_files_format_and_content: wig files
|
| |
|
| Submission date |
May 31, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Wu Wei |
| E-mail(s) |
wuwei@embl.de
|
| Organization name |
EMBL Heidelberg
|
| Department |
Genome Biology Unit
|
| Lab |
Steinmetz Lab
|
| Street address |
EMBL Heidelberg,Meyerhofstraße 1
|
| City |
Heidelberg |
| ZIP/Postal code |
69117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13272 |
| Series (2) |
| GSE38383 |
RNA polymerase II collision interrupts convergent transcription (RNA-seq) |
| GSE38384 |
RNA polymerase II collision interrupts convergent transcription |
|
| Relations |
| SRA |
SRX150906 |
| BioSample |
SAMN01001338 |
