|
| Status |
Public on Nov 30, 2012 |
| Title |
HPV+ HNSCC sample FF 39 |
| Sample type |
genomic |
| |
|
| Source name |
HNSCC
|
| Organism |
Homo sapiens |
| Characteristics |
tumour site: tonsil
tissue: HPV+ HNSCC tumor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA from fresh-frozen HNSCC patient samples was extracted using the QIAamp DNA Blood Mini Kit (QIAGEN)
|
| Label |
Biotin
|
| Label protocol |
DNA was prepared in a total volume of 20 µl (1 µg). It was then bisulphite converted using the EZ DNA Methylation kit (Zymo Research Corp, Orange, CA 92867, USA, cat No. D5001) before being hybridized to the array.
|
| |
|
| Hybridization protocol |
The Infinium HumanMethylation450 BeadChips were processed by the UCL Genomics core facility according to the manufacturer’s recommendation.
|
| Scan protocol |
The Infinium HumanMethylation450 BeadChips were processed by the UCL Genomics core facility according to the manufacturer’s recommendation.
|
| Description |
Ligated BS-converted DNA
|
| Data processing |
The scanned data and image output files were managed with the Genomestudio software (version 1.9.0) (Illumina®, Inc., San Diego, CA 92121, USA).
|
| |
|
| Submission date |
May 25, 2012 |
| Last update date |
Nov 30, 2012 |
| Contact name |
Matthias Lechner |
| E-mail(s) |
m.lechner@ucl.ac.uk
|
| Organization name |
University College London
|
| Department |
UCL Cancer Institute
|
| Lab |
Medical Genomics
|
| Street address |
Gower Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE38268 |
450k Analysis of 3 FF HPV+ and 3 FF HPV- HNSCC samples |
| GSE38271 |
FF and FFPE HPV+ and HPV- HNSCC and HNSCC cell lines |
|
