|
| Status |
Public on Apr 01, 2012 |
| Title |
whole body starvation resistant (SR) malnutritioned middle-aged biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
5 Drosophila females
|
| Organism |
Drosophila melanogaster |
| Characteristics |
natural variant/selected line: Starvation resistant
diet: malnutritioned (standard medium diluted 0.5x)
age: middle-aged (collected at 90% survival of the cohort)
tissue: whole organism
gender: female
|
| Treatment protocol |
Each sample was composed of five whole females snap-frozen in liquid nitrogen
|
| Growth protocol |
Adult virgin females were placed in vials (five individuals per vial) with media where yeast and sugar content was manipulated to obtain diet treatments representing malnutrition, optimal food and overfeeding. The concentrations of yeast and sugar were relative to those of the standard medium (20 g agar, 9 g kalmus, 10 ml nipagin, 50 g saccharose and 35 g of granulated yeast per litre water).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAwas isolated using the NucleoSpin RNA II kit (Macherey & Nagel).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 8 ug of cRNA were hybridized for 16 hr at 45C on GeneChipII Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from 5 females (whole body)
|
| Data processing |
The data analysis was performed using R (version 2.7.0) and Bioconductor. The probe level data was summarized using the robust multi-array average (RMA) algorithm.
|
| |
|
| Submission date |
Mar 16, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Agnieszka Doroszuk |
| E-mail(s) |
a.doroszuk@biology.leidenuniv.nl
|
| Organization name |
Leiden University
|
| Department |
Evolutionary Biology
|
| Street address |
Sylviusweg 72
|
| City |
Leiden |
| ZIP/Postal code |
2333 BE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE36582 |
Expression data from middle-aged and old Drosophila females |
|
| Relations |
| Reanalyzed by |
GSE119084 |
