|
| Status |
Public on Feb 17, 2012 |
| Title |
Pig5_Failure |
| Sample type |
RNA |
| |
|
| Source name |
Right ventricular free wall, heart failure
|
| Organism |
Sus scrofa |
| Characteristics |
pig number: Pig5
tissue: Right ventricular free wall
breed: Mixed breed of Hampshire, Yorkshire and Swedish Landrace
weight: 30-40 kg
treatment group: Failure
|
| Treatment protocol |
Heart failure induced by ligation of the coronary arteries on the free right ventricular wall. Heart failured was defined as right atrial pressure >20 mmHg.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was further purified using RNeasy columns (Quiagen)
|
| Label |
Biotin
|
| Label protocol |
250 nanograms of total RNA from each sample were used to prepare biotinylated fragmented cRNA according to the GeneChip® 3’ IVT Express Kit Manual (PN 702646 Rev1, Affymetrix Inc., Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® expression arrays (Porcine Genome Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Wash, Stain and Scan Manual (PN 702731 Rev2, Affymetrix Inc., Santa Clara, CA) the arrays were then washed and stained using the Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using a GeneChip® Scanner 3000 7G
|
| Description |
Gene expression data from acute ischemic heart failure sample
|
| Data processing |
The raw data was processed using Partek Genomic Suite software (Partek Inc, St Louis, MO). Two-way analysis of variance (ANOVA) method was used to calculate p values.
|
| |
|
| Submission date |
Feb 17, 2012 |
| Last update date |
Feb 17, 2012 |
| Contact name |
Per Vikholm |
| E-mail(s) |
per.vikholm@gmail.com
|
| Organization name |
Uppsala University
|
| Department |
Cardiothoracic Surgery
|
| Street address |
Akademiska sjukhuset
|
| City |
Uppsala |
| ZIP/Postal code |
751 85 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE31619 |
Right ventricular failure due to coronary artery ligation |
|
