|
| Status |
Public on Mar 29, 2015 |
| Title |
hESCs cultured on extacellular matrix and enzymatically passaged P5 total DNA methylation RepB |
| Sample type |
genomic |
| |
|
| Source name |
WA09
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
growth condition: hESCs cultured on extacellular matrix and enzymatically passagged P5
passage: P5
cell line: WA09
|
| Growth protocol |
Human Embryonic Stem Cells grew in different subtrates (Feeder Layer and extracellular matrix) and were passaged with different methods (mechanically and enzymatically)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA was extracted using Quiagen DNeasy Blood & Tissue Kit
|
| Label |
biotin and dinitrophenyl
|
| Label protocol |
C and G nucleotides are biotin-labeled; A and T nucleotides are dinitrophenyl labeled
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Biological replicate B
|
| Data processing |
The data were filtered for detection pValue < 0.01 and background subtraction was applied using Genome Studio
|
| |
|
| Submission date |
Jan 10, 2012 |
| Last update date |
Mar 29, 2015 |
| Contact name |
Francesca Boscolo |
| E-mail(s) |
francesca.boscolo@gmail.com
|
| Organization name |
UCSD
|
| Department |
Reproductive Medicine
|
| Lab |
Marianna Alperin
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92122 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE34982 |
Increased Risk of Genetic and Epigenetic Instability in Human Embryonic Stem Cells Associated with Specific Culture Conditions I |
| GSE56851 |
Increased Risk of Genetic and Epigenetic Instability in Human Embryonic Stem Cells Associated with Specific Culture Conditions |
|
