|
| Status |
Public on Jan 05, 2012 |
| Title |
HUVEC donor 4_CD34-negative fraction |
| Sample type |
RNA |
| |
|
| Source name |
HUVEC_CD34-negative fraction
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: donor 4
cell type: cultured HUVEC cells
passage: passage 3
|
| Treatment protocol |
Primary HUVEC cells were cultured up untill passage 3, isolated and then FACS-sorted by CD34 and divided in CD34-positive and CD34-negative fractions.
|
| Growth protocol |
Primary HUVECs were isolated from umbilical veins by collagenase digestion and routinely grown in M199 basal medium supplemented with 10% human serum, 10% fetal bovine serum and 1% penicillin-streptomycin-glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were labeled using the Affymetrix GeneChip 3’ IVT Express kit.
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix HT HG-133+PM 24 Array Plate GeneChips using the Affymetrix standard hybridization protocol.
|
| Scan protocol |
Array Plates were scanned using a Gene Titan System.
|
| Description |
Donor4_CD34-NEG
|
| Data processing |
Data was normalized using the RMA method included in the Affy R Bioconductor package.
|
| |
|
| Submission date |
Jan 04, 2012 |
| Last update date |
Jan 05, 2012 |
| Contact name |
Ingeborg Klaassen |
| E-mail(s) |
i.klaassen@amc.uva.nl
|
| Phone |
+31205664958
|
| Organization name |
AMC
|
| Department |
Ophthalmology
|
| Lab |
Ocular Angiogenesis Group
|
| Street address |
Meibergdreef 15
|
| City |
Amsterdam |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE34850 |
CD34 marks angiogenic tip cells in human vascular endothelial cell cultures. |
|
