|
Status |
Public on Jan 05, 2012 |
Title |
HUVEC donor 2_CD34-negative fraction |
Sample type |
RNA |
|
|
Source name |
HUVEC_CD34-negative fraction
|
Organism |
Homo sapiens |
Characteristics |
donor id: donor 2 cell type: cultured HUVEC cells passage: passage 3
|
Treatment protocol |
Primary HUVEC cells were cultured up untill passage 3, isolated and then FACS-sorted by CD34 and divided in CD34-positive and CD34-negative fractions.
|
Growth protocol |
Primary HUVECs were isolated from umbilical veins by collagenase digestion and routinely grown in M199 basal medium supplemented with 10% human serum, 10% fetal bovine serum and 1% penicillin-streptomycin-glutamine.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Samples were labeled using the Affymetrix GeneChip 3’ IVT Express kit.
|
|
|
Hybridization protocol |
Samples were hybridized to Affymetrix HT HG-133+PM 24 Array Plate GeneChips using the Affymetrix standard hybridization protocol.
|
Scan protocol |
Array Plates were scanned using a Gene Titan System.
|
Description |
Donor2_CD34-NEG
|
Data processing |
Data was normalized using the RMA method included in the Affy R Bioconductor package.
|
|
|
Submission date |
Jan 04, 2012 |
Last update date |
Jan 05, 2012 |
Contact name |
Ingeborg Klaassen |
E-mail(s) |
i.klaassen@amc.uva.nl
|
Phone |
+31205664958
|
Organization name |
AMC
|
Department |
Ophthalmology
|
Lab |
Ocular Angiogenesis Group
|
Street address |
Meibergdreef 15
|
City |
Amsterdam |
ZIP/Postal code |
1105 AZ |
Country |
Netherlands |
|
|
Platform ID |
GPL13158 |
Series (1) |
GSE34850 |
CD34 marks angiogenic tip cells in human vascular endothelial cell cultures. |
|