|
| Status |
Public on Dec 22, 2011 |
| Title |
NA_CD3__CB_2 |
| Sample type |
genomic |
| |
|
| Source name |
gDNA from CD4+ cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: neonatal CD4+
gender: mixed
age: 0
disease state: NORM
treatment: CD3
pool id: E
|
| Treatment protocol |
Anti-CD3 (0.5ug/mL) + rhIL2 (20Units) or UNTREATED
|
| Growth protocol |
cells cultured for 24 hours in AIM-V media + 2Mercapto ethanol (serum free)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA and RNA co purified from source according to Qiagen all prep kit protocol
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Cy5 and Cy3
|
| |
|
| Hybridization protocol |
Infinium Methylation Assay according to manufacturers protocol (Illumina)
|
| Scan protocol |
iScan system
|
| Description |
neonatal CD4+
|
| Data processing |
Genome studio v2010.3 with Methylation Module v1.8.5 under default settings
|
| |
|
| Submission date |
Dec 21, 2011 |
| Last update date |
Dec 22, 2011 |
| Contact name |
David Martino |
| E-mail(s) |
david.martino@telethonkids.org.au
|
| Phone |
0863191635
|
| Organization name |
Telethon Kids Institute
|
| Street address |
Perth Childrens Hospital, 15 Hospital Avenue
|
| City |
NEDLANDS |
| State/province |
Western Australia |
| ZIP/Postal code |
6009 |
| Country |
Australia |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE34639 |
Methylation data from CD4+ cells at birth and 12 months from a cohort of children who were diagnosed with IgE food allergy to either egg milk or peanut at 12 months (n=12) and healthy controls (n=12). |
|
