|
| Status |
Public on Dec 14, 2011 |
| Title |
Day 70 T2 Control, Biological Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Day 70 T2 Control
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: Male
tissue: Whole Brain (with olfactory bulb)
treament: Matched Control
model: T2
# samples pooled: 3
|
| Treatment protocol |
Day 70 male mice were subjected to CO2 asphyxiation followed by cervical disolaction. Whole brain tissue was dissected in under five minutes and flash frozen liquid nitrogen.
|
| Growth protocol |
C57BL/6J mice were prenatally exposed to ethanol either via Trimister 1,2, or 3, injections or continous preference drinking (Labelled T1, T2, T3, or CPD, respectively).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol according to manufacturers protocol
|
| Label |
Biotin
|
| Label protocol |
One microgram of total RNA was labeled using the Flash Tag Biotin HSR kit (Genisphere)
|
| |
|
| Hybridization protocol |
Samples were then hybridized to Affymetrix miRNA 2.0 arrays for 16 hours at 45°C
|
| Scan protocol |
Probe level (.CEL file) data was generated using Affymetrix Command Console v1.1.
|
| Description |
miRNA expression data from day 70 matched control malesdu ring Trimester 2 (GD 14 and 16) via maternal injections of 0.15M saline with a dosage of 2.5g/kg. Saline was administered twice separated by two hours.
|
| Data processing |
Probes were summarized to gene level data in Partek Genomics Suite v6.6 (Partek, St. Louis, MO, USA) using the RMA algorithm. Partek software was used to determine ANOVA p-values and fold changes.
|
| |
|
| Submission date |
Dec 13, 2011 |
| Last update date |
Dec 14, 2011 |
| Contact name |
Benjamin I Laufer |
| Organization name |
Genentech
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
California |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE34413 |
Persistent Alterations in the imprinted miRNA and snoRNA populations in mouse models of fetal alcohol spectrum disorders (FASD) |
|
