|
| Status |
Public on May 16, 2024 |
| Title |
N87_shNC_1 |
| Sample type |
SRA |
| |
|
| Source name |
NCI-N87
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI-N87
cell type: human gastric cancer cells
treatment: shNC
|
| Treatment protocol |
Cells were grown overnight to a density of 1×105 cells/well, then infected with viral supernatant at a multiplicity of infection of 10. To isolate stable clones, puromycin (2 μg/ml, Sigma) was utilized for a minimum duration of 1 week.
|
| Growth protocol |
NCI-N87 human gastric cancer cells were maintained in RMPI1640 supplemented with 10%FBS(Fetal Bovine Serum) and antibiotics in humidfied atmosphere with 5% CO2 at 37℃
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit(Qiagen).1.3ug of tatol RNA was used for the construction of sequencing libraries
RNA libraries for RNA-seq were prepared using SMATER Mrna-Seq Library Prep Kit following manufacturer's protocls
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Analyzing Sequence Quality with FastQC
Removing low quality sequences with Trim_Galore
Genome Comparison with STAR-aligner
Summarize gene counts with featureCounts
Generate an allegation analysis report using multiQC
Assembly: not provided
Supplementary files format and content: tab-delimiited text file includes raw counts, RPKM for each Sample
|
| |
|
| Submission date |
May 12, 2024 |
| Last update date |
May 16, 2024 |
| Contact name |
Sheng-Ze long Wu |
| E-mail(s) |
woosze66@outlook.com
|
| Phone |
13358412365
|
| Organization name |
Concord Hospital of the Fujian Medical University
|
| Street address |
NO.29,XINQUAN ROAD,FUZHOU 350001 FUJIAN PROVINCE,CHINA.
|
| City |
Fuzhou |
| State/province |
None Selected |
| ZIP/Postal code |
350000 |
| Country |
China |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE267263 |
Increased ONECUT2 induced by Helicobacter pylori promotes gastric cancer cell stemness via AKT-related pathway |
|
| Relations |
| BioSample |
SAMN41376928 |
| SRA |
SRX24530627 |
