|
| Status |
Public on Apr 10, 2012 |
| Title |
17955 L |
| Sample type |
RNA |
| |
|
| Source name |
Dermal lymphatic endothelial cells, buttock
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 22 years
tissue: buttock
cell type: Dermal lymphatic endothelial cells
|
| Growth protocol |
Endothelial cells were grown in EGM-2 MV contained Endothelial Cell Basal Medium-2 (EBM-2) supplemented with 25 ml FBS, 0.2 ml hydrocortisone, 2 ml hFGF, 0.5 ml VEGF, 0.5 ml R3-IGF-1, 0.5 ml ascorbic acid, 0.5 ml hEGF, and 0.5 ml GA-1000 (all Lonza).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Endothelial cells were trypsinized and total cellular RNA was isolated after centrifugation using the RNeasy Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
100 ng of each RNA was used as template to produce Cy3- labeled cRNA. The RNA samples were amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Cy3 labeled cRNAs were hybridized overnight (17 hours, 65°C) to Agilent Whole Human Genome Oligo Microarrays (44K) using Agilent's recommended hybridization chamber and oven.Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
|
| Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
|
| Description |
punch biopsy
SAMPLE 10
|
| Data processing |
The Agilent Feature Extraction Software (FES 10.5.1.1 ) was used to read out and process the microarray image files.
|
| |
|
| Submission date |
Oct 10, 2011 |
| Last update date |
Apr 10, 2012 |
| Contact name |
Silvia Rueberg |
| Organization name |
Miltenyi Biotec GmbH
|
| Department |
Genomic Services Department
|
| Street address |
Friedrich-Ebert-Str. 68
|
| City |
Bergisch Gladbach |
| ZIP/Postal code |
51429 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE32709 |
DNA methylation regulates lineage-specifying genes in the human vascular system [expression array]. |
| GSE34487 |
DNA methylation regulates lineage-specifying genes in the human vascular system. |
|
