|
| Status |
Public on May 01, 2024 |
| Title |
Control WML 4 |
| Sample type |
RNA |
| |
|
| Source name |
Human post-mortem tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age (yrs): 84
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the LCM-isolated cells using the PicoPure RNA isolation kit (Life technologies: Applied Biosystems, USA).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Approximately 5.5 μg amplified cDNA was fragmented, labelled and hybridised to Clariom S Array chips for 16 h at 45 °C in a rotating oven at 60 rpm.
|
| Scan protocol |
Using the Fluidics Station 400 and GeneChip Operating System, a series of washing steps followed to remove any unbound DNA, before each microarray was stained and scanned using the GeneChip 3000 scanner.
|
| Description |
Control case
|
| Data processing |
Affymetrix Expression Console software (Affymetrix®, Stockport, UK) was employed to quality control the data and principal component analysis (PCA) in Qlucore Omics Explorer software (Qlucore, Lund, Sweden) was used to visually inspect the data and identify sample outliers. Transcriptome Analysis Console (TAC) software, version 4.1.1 (Affymetrix®, UK) was used to analyse and compare the gene expression profile of the 3 groups.
|
| |
|
| Submission date |
Mar 04, 2024 |
| Last update date |
May 01, 2024 |
| Contact name |
Rachel Waller |
| E-mail(s) |
r.waller@sheffield.ac.uk
|
| Organization name |
The University of Sheffield
|
| Department |
Neuroscience
|
| Street address |
SITraN, 385A Glossop Road
|
| City |
SHEFFIELD |
| State/province |
South Yorkshire |
| ZIP/Postal code |
S102HQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE260815 |
The microglial transcriptome of age-associated deep subcortical white matter lesions suggests a neuroprotective response to blood-brain barrier dysfunction (microarray) |
|
