NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM799863 Query DataSets for GSM799863
Status Public on Jun 10, 2012
Title HCT116_DKO_WGA_rep6_glioblstoma study
Sample type genomic
 
Source name Genomic DNA from HCT116 DNMT1;DNMT3 double knockout cell line
Organism Homo sapiens
Characteristics cell line: HCT116 DNMT1;DNMT3 double knockout
idh1/2 genotype: wildtype
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using the QIAamp DNA mini kit, according to the manufacturer's protocol
Label Cy5 and Cy3
Label protocol Standard Illumina protocol
 
Hybridization protocol Genomic DNA was bisulfite converted with the Zymo Research EZ DNA Methylation kit. Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol BeadChips were imaged with the Illumina HiScan scanner using standard settings
Data processing Mean background-corrected and normalized signal intensities of the methylation (M) and unmethylated (U) estimates for each CpG locus were extracted using Illumina BeadStudio software
 
Submission date Sep 21, 2011
Last update date Jun 10, 2012
Contact name Derek Chiang
E-mail(s) chiang@med.unc.edu
Organization name University of North Carolina
Department Department of Genetics
Street address UNC Lineberger Cancer Center, 450 West Drive, CB #7295
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7295
Country USA
 
Platform ID GPL13534
Series (2)
GSE32283 Mutations in IDH1 are associated with DNA hypermethylation in glioblastomas
GSE32286 DNA hypermethylation in intrahepatic cholangiocarcinomas and glioblastomas

Data table header descriptions
ID_REF
VALUE Average Beta
L06_Unmethyl.Detection.Pval

Data table
ID_REF VALUE L06_Unmethyl.Detection.Pval
cg00000029 0.03414494 0
cg00000108 0.07099913 0
cg00000109 0.07251375 0
cg00000165 0.08050515 0
cg00000236 0.08329357 0
cg00000289 0.1625087 0
cg00000292 0.05812414 0
cg00000321 0.02538596 0
cg00000363 0.1077514 0
cg00000622 0.01832037 0
cg00000658 0.06477807 0
cg00000714 0.05666912 0
cg00000721 0.08773499 0
cg00000734 0.08166579 0
cg00000769 0.03653507 0
cg00000807 0.0797678 0
cg00000884 0.07057714 0
cg00000905 0.1052736 0
cg00000924 0.0551365 0
cg00000948 0.2137123 0

Total number of rows: 485577

Table truncated, full table size 11099 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap