|
Status |
Public on Jan 31, 2024 |
Title |
genomic DNA from posterior fossa type A epenymoma R11 primary |
Sample type |
genomic |
|
|
Source name |
Posterior fossa
|
Organism |
Homo sapiens |
Characteristics |
who-grade: N/A age (years): N/A Sex: N/A extent of resectoin: N/A material: FFPE time to first relapse (years): 2.88
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was isolated from FFPE tissue using the ReliaPrep™ FFPE gDNA Miniprep System (Promega) according to manufacturer’s instructions.
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
100-500 ng DNA were used for bisulfite conversion with the EZ DNA Methylation Kit (Zymo Research). Afterwards, the DNA Clean & Concentrator-5 (Zymo Research) and the Infinium HD FFPE DNA Restore Kit (Illumina) were used to clean and restore the converted DNA.
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
R11_primary 206359150140_R04C01
|
Data processing |
R (v4.2.2)
|
|
|
Submission date |
Nov 26, 2023 |
Last update date |
Jan 31, 2024 |
Contact name |
Swenja Gödicke |
Organization name |
Research Institute Children‘s Cancer Center Hamburg
|
Lab |
Schüller
|
Street address |
Martinistrasse 52
|
City |
Hamburg |
ZIP/Postal code |
20251 |
Country |
Germany |
|
|
Platform ID |
GPL21145 |
Series (2) |
GSE248617 |
PFA ependymoma primary and recurring tumors show differences in cellularity, DNA methylation and relevant copy number variations. |
GSE248811 |
Clinically relevant molecular hallmarks of PFA ependymomas display intratumoral heterogeneity and correlate with tumor morphology. |
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