|
| Status |
Public on Jan 31, 2024 |
| Title |
genomic DNA from posterior fossa type A epenymoma R7 relapse |
| Sample type |
genomic |
| |
|
| Source name |
Posterior fossa
|
| Organism |
Homo sapiens |
| Characteristics |
who-grade: 3
age (years): 3.0
Sex: m
extent of resectoin: STR
material: FFPE
time to first relapse (years): 2.91
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated from FFPE tissue using the ReliaPrep™ FFPE gDNA Miniprep System (Promega) according to manufacturer’s instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
100-500 ng DNA were used for bisulfite conversion with the EZ DNA Methylation Kit (Zymo Research). Afterwards, the DNA Clean & Concentrator-5 (Zymo Research) and the Infinium HD FFPE DNA Restore Kit (Illumina) were used to clean and restore the converted DNA.
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
R7_relapse
205756370079_R05C01
|
| Data processing |
R (v4.2.2)
|
| |
|
| Submission date |
Nov 26, 2023 |
| Last update date |
Jan 31, 2024 |
| Contact name |
Swenja Gödicke |
| Organization name |
Research Institute Children‘s Cancer Center Hamburg
|
| Lab |
Schüller
|
| Street address |
Martinistrasse 52
|
| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21145 |
| Series (2) |
| GSE248617 |
PFA ependymoma primary and recurring tumors show differences in cellularity, DNA methylation and relevant copy number variations. |
| GSE248811 |
Clinically relevant molecular hallmarks of PFA ependymomas display intratumoral heterogeneity and correlate with tumor morphology. |
|
