|
| Status |
Public on Oct 05, 2005 |
| Title |
GM-CSF macrophage with BCG stimulation/CD14 PMNC slide B, first trial |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
GM-CSF macrophage with BCG stimulation
|
| Organism |
Homo sapiens |
| Characteristics |
Culture of CD14 cells with GM-CSF for 7 days and 24 hours stimulation with BCG.
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
CD14 PMNC
|
| Organism |
Homo sapiens |
| Characteristics |
CD14 cells were seperated from PBMN by magnetic sorting.
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Description |
RNA amplified before cDNA synthesis.
|
| Data processing |
Lowess normalization (GeneSpring software)
|
| |
|
| Submission date |
Oct 04, 2005 |
| Last update date |
Jul 27, 2006 |
| Contact name |
Vahid Khajoee |
| E-mail(s) |
vkhajoee@pediatr.med.kyushu-u.ac.jp
|
| Organization name |
Kyuhsu University
|
| Department |
Pediatrics
|
| Street address |
3-1-1 Maidashi, higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1294 |
| Series (1) |
| GSE3408 |
Novel Roles of Osteopontin and CXC Chemokine Ligand 7 in the Defense against Mycobacterial Infection |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
same as UNF_VALUE but with flagged values removed |
| Spot labels |
Physical place of spots on slide B. |
| ARM Dens - ALU / mm2-CH1 |
Artifact-removed density value for each spot for Cy3 signal (GM-CSF macrophage with BCG stimulation). |
| Bkgd-CH1 |
Background density or volume for Cy3 signal (GM-CSF macrophage with BCG stimulation). |
| sARMDens-CH1 |
ARM Density value of the spot, minus the background Density value for Cy3 signal (GM-CSF macrophage with BCG stimulation). |
| S/N-CH1 |
Spot density minus Background density, divided by the SD of the Background density for Cy3 signal. |
| Flag-CH1 |
The value is 0 when there is no dust and 1 when dust can be seen for spots from Cy3 signal. |
| ARM Dens - ALU / mm2-CH2 |
Artifact-removed density value for each spot for Cy5 signal (CD14 PMNC). |
| Bkgd-CH2 |
Background density or volume for Cy5 signal (CD14 PMNC). |
| sARMDens-CH2 |
RM Density value of the spot, minus the background Density value for Cy5 signal (CD14 PMNC). |
| S/N-CH2 |
Spot density minus Background density, divided by the SD of the Background density for Cy5 signal. |
| Flag-CH2 |
The value is 0 when there is no dust and 1 when dust can be seen for spots from Cy5 signal. |
| UNF_VALUE |
Log ratio of normalized data for slide B (GM-CSF macrophage with BCG stimulation/CD14 PMNC). |
