|
| Status |
Public on May 14, 2012 |
| Title |
ES_MEL2_passage15_____Female_36.133.2.1 |
| Sample type |
RNA |
| |
|
| Source name |
ES_MEL2_passage15_____Female_36.133.2.1
|
| Organism |
Homo sapiens |
| Characteristics |
loring pipeline sample id: 36.133.2.1
loring pipeline stem cell matrix version: stem cell matrix version 342
cell type: embryonic stem cell, undifferentiated
cell line: MEL2
passage number: passage15
gender: Female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the MirVana RNA extraction kit (Ambion), quantified using the Ribogreen reagent (Lifetech, Inc.), and quality-controlled on a Bioanalyzer (Agilent). Two hundred nanograms of input total RNA was amplified and labeled using the TotalPrep kit (Ambion). The labeled product was then hybridized to Illumina HT12 arrays and scanned on a BeadArray Reader (Illumina, Inc.) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
ES_F_MEL2P15_54
|
| Data processing |
The data were normalized by RSN normalization using LUMI package in R. Some probes excluded because of probe failure/poor performance.
|
| |
|
| Submission date |
Jul 13, 2011 |
| Last update date |
Apr 03, 2013 |
| Contact name |
Kristopher L. Nazor |
| E-mail(s) |
kristopher.nazor@gmail.com
|
| Organization name |
The Scripps Research Institute
|
| Department |
Regenerative Medicine
|
| Lab |
Loring
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE30652 |
Recurrent Variations in DNA Methylation in Human Pluripotent Stem Cells and their Differentiated Derivatives [Illumina HT12v3 Gene Expression] |
| GSE30654 |
Recurrent Variations in DNA Methylation in Human Pluripotent Stem Cells and their Differentiated Derivatives |
|
