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| Status |
Public on Jul 03, 2023 |
| Title |
hNSC treated with TCQA_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Human neural stem cells treated with 10uM TCQA for 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell: Human Neural Stem Cell
|
| Treatment protocol |
The hNSCs were seeded in 60-mm Petri dishes at the density of 2.5×104 cells/cm2 and were incubated at 37ºC for 48 h. After that, cells were treated with differentiation medium with or without 10 μM of TCQA and were incubated at 37ºC for 24 h.
|
| Growth protocol |
The hNSCs derived from H9 hNSCs were purchased from Gibco, Japan. The hNSCs were cultured in 60 mm Petri dishes (BD Falcon) with a growth medium Knock OutTM:1:1 (v/v) mixture of Dulbecco’s minimum essential medium (Sigma, U.S.A.) and Ham’s F-12 nutrient mixture (Sigma, U.S.A.) supplemented with 2% StemPro neural supplement, 20 ng/mL of fibroblast growth factor (FGF) basic recombinant human, 20 ng/mL epidermal growth factor (EGF) recombinant human and 2 mM GlutaMAX-I supplement. For adhesion, 60-mm Petri dish coated with CELLStart were used. The hNSCs were cultured at 37ºC in a 95% air / 5% CO2 humidified incubator. The medium was changed every two days to keep cells undifferentiated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted from the cells using ISOGEN kit (Nippon Gene Co. Ltd., Toyama, Japan) following manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled amplified RNA (aRNA) was synthesized by in vitro transcription using the GeneChip 3’ IVT Express Kit (Affymetrix Inc., Santa Clara, CA, USA).
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| |
|
| Hybridization protocol |
Purified and fragmented aRNA was hybridized using the GeneChip MG-430 PM microarray (Affymetrix Inc., Santa Clara, CA, USA).
|
| Scan protocol |
Scanning was performed with the GeneAtlas Imaging Station (Affymetrix Inc., Santa Clara, CA, USA).
|
| Description |
Gene expression data from human neural stem cells treated with 10uM TCQA for 24 hours
|
| Data processing |
Data analysis was performed using the Partek Express software (Partek Inc., St. Louis, MO, USA) provided by Affymetrix as part of their GeneAtlas system. Compared with the non-treated cells, fold change in expression in the TCQA-treated group was calculated and converted to log 2 data.
RMA
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| |
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| Submission date |
Jun 26, 2023 |
| Last update date |
Jul 03, 2023 |
| Contact name |
Ashley Chen |
| E-mail(s) |
ashleychen2009@gmail.com
|
| Organization name |
University of Tsukuba
|
| Street address |
1 Chome-1-1 Tennodai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8577 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE235835 |
Gene expression data from human neural stem cells (hNSCs) treated with 10 uM TCQA for 24 hours |
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