GEO help: Mouse over screen elements for information.
Status
Public on Sep 21, 2005
Title
Cell culture. A549 treated by MK886, control 4
Sample type
RNA
Source name
cell culture
Organism
Homo sapiens Characteristics
Extracted molecule
total RNA
Extraction protocol
The cells were treated by the drug or by the solvent control, tripsinized and lysed in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 8 ?g of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
cell culture
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Sep 19, 2005
Last update date
Aug 28, 2018
Contact name
Anatoly L. Mayburd
E-mail(s)
mayburna@mail.nih.gov Phone
1-301-402-3308
Organization name
NIH/NCI
Department
Intervention section
Lab
Mulshine
Street address
9000 Rockville Pyke
City
Bethesda
State/province
MD
ZIP/Postal code
20892
Country
USA
Platform ID
GPL570 Series (1)
GSE3202 MK886 treatment of H720 non-small cell lung cancer cell line
Relations Reanalyzed by
GSE64985 Reanalyzed by
GSE119087
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
1564.5
P
0.000754
AFFX-BioB-M_at
2326.1
P
0.00011
AFFX-BioB-3_at
1412
P
0.000169
AFFX-BioC-5_at
3420.1
P
0.000509
AFFX-BioC-3_at
2745.9
P
0.000044
AFFX-BioDn-5_at
3960
P
0.000044
AFFX-BioDn-3_at
16052.7
P
0.00006
AFFX-CreX-5_at
50990
P
0.000052
AFFX-CreX-3_at
58334.2
P
0.000044
AFFX-DapX-5_at
11.8
A
0.672921
AFFX-DapX-M_at
73.7
A
0.544587
AFFX-DapX-3_at
48.1
A
0.39692
AFFX-LysX-5_at
109.4
A
0.139482
AFFX-LysX-M_at
91.8
A
0.631562
AFFX-LysX-3_at
61.9
A
0.382599
AFFX-PheX-5_at
17.4
A
0.804734
AFFX-PheX-M_at
5.9
A
0.883887
AFFX-PheX-3_at
29.9
A
0.834139
AFFX-ThrX-5_at
28.5
A
0.824672
AFFX-ThrX-M_at
11.2
A
0.760956
54675 1450 Kbytes .Supplementary file Size Download File type/resource GSM75015.CEL.gz
7.6 Mb
(ftp) (http) CEL
GSM75015.CHP.gz
12.7 Mb
(ftp) (http) CHP
GSM75015.dat.gz
94.0 Mb
(ftp) (http) DAT
Processed data provided as supplementary file
