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Sample GSM746713 Query DataSets for GSM746713
Status Public on Jan 01, 2012
Title OsDIS1 Ovexpression plants_ drought conditions_rep1
Sample type RNA
 
Source name OsDIS1 overexpression rice leaves,drought conditions, replicate 1
Organism Oryza sativa
Characteristics genotype/variation: OsDIS1 overexpression
stress: drought
tissue: Leaves
age: Seven-day-old
Treatment protocol The OsDIS1 overexpression 9-4-2 plants and the wild-type plants were cultured on 1/2 MS medium plus 3% sucrose for seven days. About half of the plants were sampled as the untreated control for RNA isolation, and the rest were transferred with 1/2 MS medium onto filter papers to induce drought stress. When the leaves of the OsDIS1 overexpression plants began to show drought stress phenotypes, we collected leaves for RNA isolation
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIZOL Reagent (Cat#15596-018,Life technologies, Carlsbad, CA, US)following the manufacturer's instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).
Label Cy3
Label protocol Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat#5190-2305, Agilent technologies, Santa Clara, CA, US), followed the manufacturer's instructions. Labeled cRNA were purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany).
 
Hybridization protocol Each Slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat#5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat#G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer's instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat#121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat#5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer's instructions。
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings,Dye channel: Green ,Scan resolution=5μm, PMT 100%,10% ,16bit.
Description D_OX 9-4-2_Log2NormSignal
251524111878_S01_GE1_105_Dec08_1_4.txt
Gene expression in OsDIS1 overexpression plants under drought conditions
Data processing Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US) Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US).
 
Submission date Jun 22, 2011
Last update date Jan 01, 2012
Contact name Joseph Ning
E-mail(s) yuese791975@163.com
Organization name Hunan Agricultural Univrsity
Lab Crop Gene Engineering Key Laboratory of Hunan Province
Street address No.1 Nongda Road,Furong District
City Changsha
State/province Hunan
ZIP/Postal code 410128
Country China
 
Platform ID GPL8852
Series (1)
GSE30151 Global gene expression analysis of the OsDIS1 overexpression plants under normal and drought stress conditions

Data table header descriptions
ID_REF
VALUE quantile normalized, log2 signal

Data table
ID_REF VALUE
Os01g0532600|mRNA|AJ491820|CDS+3'UTR 7.537098
Os01g0721700|COMBINER_EST|CI557169|4 9.313088
Os06g0215600|mRNA|AK104039|CDS+3'UTR 7.7758656
Os09g0379500|mRNA|AK069390|CDS+3'UTR 3.192852
Os03g0199100|mRNA|AK069890|CDS+3'UTR 10.77932
Os01g0508500|mRNA|AK120501|CDS+3'UTR 12.0914755
Os06g0130000|mRNA|AK064427|CDS+3'UTR 8.928135
Os08g0446400|mRNA|AK102368|5'UTR+CDS 5.026124
Os05g0433800|COMBINER_EST|Os05g0433800|8 3.5409606
Os12g0152700|mRNA|AK099473|CDS+3'UTR 15.451029
Os03g0685100|mRNA|AK059852|CDS+3'UTR 9.5599575
Os05g0285900|mRNA|AK061533|CDS+3'UTR 11.511274
Os03g0449000|COMBINER|CI260116|6 7.5291295
Os03g0775000|COMBINER_EST|AU057613|7 11.66967
Os11g0213500|COMBINER_EST|Os11g0213500|8 4.6511993
Os09g0261100|mRNA|AK121607|CDS+3'UTR 10.993272
Os02g0236600|COMBINER_EST|CI552267|0 11.408261
Os10g0469200|mRNA|AK108708|CDS+3'UTR 7.2637525
Os02g0827200|mRNA|AY137242|CDS+3'UTR 11.205936
Os09g0271000|mRNA|AK102955|CDS+3'UTR 2.8923106

Total number of rows: 42416

Table truncated, full table size 1920 Kbytes.




Supplementary file Size Download File type/resource
GSM746713.txt.gz 7.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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