|
| Status |
Public on Apr 25, 2011 |
| Title |
early atherosclerotic plaque8 |
| Sample type |
RNA |
| |
|
| Source name |
postmortem plaque
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: carotid artery
phenotype: early atherosclerotic plaque
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Guanidine isothiocyanate/CsCl method followed by further purification and concentration using RNease mini columns (Qiagenm Hilden, Germany)
|
| Label |
Biotin
|
| Label protocol |
GeneChip IVT Labeling Kit (Affymetrix, Santa Clara, USA, CA)
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol (GeneChip Expression Analysis Technical Manual)
|
| Scan protocol |
GeneChip Scanner 3000 (Affymetrix, Santa Clara, USA, CA)
|
| Description |
Early atherosclerotic plaque from human carotid (autopsy)
|
| Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
| |
|
| Submission date |
Apr 25, 2011 |
| Last update date |
Apr 25, 2011 |
| Contact name |
Marco Manca |
| E-mail(s) |
marco.manca@cern.ch, marco@scimpulse.org
|
| Organization name |
University of Maastricht
|
| Department |
CARIM
|
| Lab |
Experimental Vascular Pathology
|
| Street address |
P. Debyelaan 25
|
| City |
Maastricht |
| State/province |
Limburg |
| ZIP/Postal code |
6229 HX |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28829 |
Gene Expression in early and advanced atherosclerotic plaque from human carotid |
|
