|
| Status |
Public on Apr 11, 2012 |
| Title |
MCF-7, Insx24h-1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF_7
treatment: serum-free medium x 24 hrs, then with 10 ug/mL insulin x 24 hrs
|
| Treatment protocol |
Cells were seeded in 100 mm dishes in triplicate. The following day, cells were washed with PBS, and treated with serum-free IMEM. Twenty-four hrs later, cells were treated +/- 10 ug/mL insulin. RNA was harvested after 4 or 24 hrs of insulin stimulation.
|
| Growth protocol |
MCF-7 cells are maintained in IMEM + 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by Qiagen RNeasy column clean-up with on-column DNase digestion
|
| Label |
biotin
|
| Label protocol |
GeneChip One-Cycle Target Labeling and Control reagents kit (Affymetrix)
|
| |
|
| Hybridization protocol |
15 ug biotinylated cRNA was fragmented and hybridizied to an Affymetrix Genechip Human Genome Plus 2.0-PM array
|
| Scan protocol |
Hybridized cRNA was detected using streptavidin coupled to phycoerythrin. Genechips were scanned using Genechip Scanner 3000 7G Plus 2 and Genechip Operating System (Affymetrix)
|
| Description |
Gene expression data from cells treated with serum-free medium x 24 hrs, then with 10 ug/mL insulin x 24 hrs
|
| Data processing |
Default values were used to grid images (.DAT) and generate .CEL and .CHP files.
|
| |
|
| Submission date |
Apr 13, 2011 |
| Last update date |
Apr 11, 2012 |
| Contact name |
Todd W Miller |
| E-mail(s) |
todd.miller@vanderbilt.edu
|
| Organization name |
Vanderbilt Univ
|
| Department |
Medicine
|
| Street address |
2220 Pierce Ave, 777 PRB
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37174 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE28580 |
RNA interference screening identifies the Insulin/IGF-1 receptor pathway as a mechanism of escape from hormone dependence in breast cancer |
|
